PDBsum entry 6x0c

Lyase

PDB id: 6x0c

Contents

Protein chains

253 a.a.

394 a.a.

Ligands

BZI ×3

EDO ×2

PEG ×2

DMS ×6

0JO

_CS ×2

Metals

_CL ×3

Waters ×608

PDB id:

6x0c

Name:

Lyase

Title:

Tryptophan synthase mutant beta-q114a in complex with cesium ion at the metal coordination site and aminoacrylate and benzimidazole at the enzyme beta site

Structure:

Tryptophan synthase alpha chain. Chain: a. Engineered: yes. Tryptophan synthase beta chain. Chain: b. Engineered: yes. Mutation: yes

Source:

Salmonella typhimurium (strain lt2 / sgsc1412 / atcc 700720). Organism_taxid: 99287. Strain: lt2 / sgsc1412 / atcc 700720. Gene: trpa, stm1727. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: trpb, stm1726.

Resolution:

1.45Å R-factor: 0.166 R-free: 0.202

Authors:

E.Hilario,M.F.Dunn,L.J.Mueller,L.Fan

Key ref:

E.Hilario et al. External aldimine form of tryptophan synthase mutant beta-Q114a in complex with cesium ion at the metal coordination site and benzimidazole at the enzyme bet. To be published, .

Date:

15-May-20 Release date: 10-Mar-21

Protein chain

P00929  (TRPA_SALTY) -  Tryptophan synthase alpha chain from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

Seq: 268 a.a.

Struc: 253 a.a.

Protein chain

P0A2K1  (TRPB_SALTY) -  Tryptophan synthase beta chain from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

Seq: 397 a.a.

Struc: 394 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: Chains A, B: E.C.4.2.1.20 - tryptophan synthase.
• Pathway: Tryptophan Biosynthesis
• Reaction: (1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-glyceraldehyde 3-phosphate + L-tryptophan + H2O

(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-glyceraldehyde 3-phosphate (Bound ligand (Het Group name = EDO) matches with 40.00% similarity) + L-tryptophan + H2O

• Cofactor: Pyridoxal 5'-phosphate

Pyridoxal 5'-phosphate (Bound ligand (Het Group name = 0JO) matches with 68.18% similarity)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site