PDBsum entry 6w9c

Hydrolase

PDB id: 6w9c

Contents

Protein chains

310 a.a.

Metals

_CL ×3

_ZN ×4

PDB id:

6w9c

Name:

Hydrolase

Title:

The crystal structure of papain-like protease of sars cov-2

Structure:

Non-structural protein 3. Chain: a, b, c. Synonym: nsp3,pl2-pro,papain-like protease,papain-like proteinase,pl- pro. Engineered: yes. Other_details: two c-terminal alanine residues are cloning artifacts

Source:

Severe acute respiratory syndrome coronavirus 2. 2019-ncov. Organism_taxid: 2697049. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

2.70Å R-factor: 0.237 R-free: 0.279

Authors:

J.Osipiuk,R.Jedrzejczak,C.Tesar,M.Endres,L.Stols,G.Babnigg,Y.Kim, K.Michalska,A.Joachimiak,Center For Structural Genomics Of Infectious Diseases (Csgid)

Key ref:

J.Osipiuk et al. The crystal structure of papain-Like protease of sars. To be published, .

Date:

22-Mar-20 Release date: 01-Apr-20

Protein chains

P0DTD1  (R1AB_SARS2) -

Enzyme reactions

• Enzyme class: E.C.3.4.19.12 - ubiquitinyl hydrolase 1.
• Reaction: Thiol-dependent hydrolysis of ester, thiolester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).