PDBsum entry 6vpm

Transferase

PDB id: 6vpm

Contents

Protein chains

277 a.a.

Ligands

AMP ×2

R74 ×2

MLA ×2

Metals

_MG ×2

Waters ×502

PDB id:

6vpm

Name:

Transferase

Title:

Tpx2 residues 7-20 fused to aurora a residues 116-389 with c290 disulfide bonded to compound 8-34, and in complex with amp-pnp

Structure:

Tpx2 fragment - aurora a kinase domain fusion. Chain: a, b. Fragment: kinase domain. Synonym: targeting protein for xklp2,aurora kinase a,differentially expressed in cancerous and non-cancerous lung cells 2,dil-2, hepatocellular carcinoma-associated antigen 519,hepatocellular carcinoma-associated antigen 90,protein fls353,restricted expression proliferation-associated protein 100,p100,aurora 2,aurora/ipl1- related kinase 1,hark1,breast tumor-amplified kinase,

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: tpx2, c20orf1, c20orf2, dil2, hca519, aurka, aik, airk1, ark1, aura, ayk1, btak, iak1, stk15, stk6. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.58Å R-factor: 0.170 R-free: 0.203

Authors:

D.C.Lim,M.B.Yaffe

Key ref:

D.C.Lim et al. (2020). Redox priming promotes aurora a activation during mit. Sci signal, 13, . PubMed id: 32694171

Date:

03-Feb-20 Release date: 05-Aug-20

Protein chains

O14965  (AURKA_HUMAN) -  Aurora kinase A from Homo sapiens

Seq: 403 a.a.

Struc: 277 a.a.*

Protein chains

Q9ULW0  (TPX2_HUMAN) -  Targeting protein for Xklp2 from Homo sapiens

Seq: 747 a.a.

Struc: 277 a.a.*

* PDB and UniProt seqs differ at 258 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.2.7.11.1 - non-specific serine/threonine protein kinase.
• Reaction:
  1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
  2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺

L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] (Bound ligand (Het Group name = AMP) matches with 85.19% similarity) + ADP + H(+)

L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] (Bound ligand (Het Group name = AMP) matches with 85.19% similarity) + ADP + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site