PDBsum entry 6uhd

Transferase

PDB id: 6uhd

Contents

Protein chains

322 a.a.

15 a.a.

14 a.a.

Ligands

ACT

CO6 ×2

GOL ×3

SO4 ×2

UNX ×16

Waters ×746

PDB id:

6uhd

Name:

Transferase

Title:

Crystal structure of human histone acetyltransferase 1 (hat1) in complex with isobutyryl-coa and k12a mutant variant of histone peptide h4

Structure:

Histone acetyltransferase type b catalytic subunit. Chain: a, b. Synonym: histone acetyltransferase 1. Engineered: yes. Histone h4. Chain: c, d. Engineered: yes. Mutation: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: hat1, kat1. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Expression_system_variant: -prare2. Synthetic: yes. Organism_taxid: 9606

Resolution:

1.60Å R-factor: 0.174 R-free: 0.199

Authors:

L.Halabelian,H.Zeng,A.Dong,P.Loppnau,C.Bountra,A.M.Edwards, C.H.Arrowsmith,Structural Genomics Consortium (Sgc)

Key ref:

L.Halabelian et al. Crystal structure of human histone acetyltransferase (hat1) in complex with isobutyryl-Coa and k12a mutant variant of histone peptide h4. To be published, .

Date:

27-Sep-19 Release date: 16-Oct-19

Protein chains

O14929  (HAT1_HUMAN) -  Histone acetyltransferase type B catalytic subunit

Seq: 419 a.a.

Struc: 322 a.a.*

Protein chain

P62805  (H4_HUMAN) -  Histone H4

Seq: 103 a.a.

Struc: 15 a.a.*

Protein chain

P62805  (H4_HUMAN) -  Histone H4

Seq: 103 a.a.

Struc: 14 a.a.*

* PDB and UniProt seqs differ at 5 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chains A, B: E.C.2.3.1.48 - histone acetyltransferase.
• Reaction: L-lysyl-[protein] + acetyl-CoA = N₆-acetyl-L-lysyl-[protein] + CoA + H⁺

L-lysyl-[protein] (Bound ligand (Het Group name = CO6) matches with 96.23% similarity) + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site