PDBsum entry 6u5h

Unknown function

PDB id: 6u5h

Contents

Protein chains

318 a.a.

PDB id:

6u5h

Name:

Unknown function

Title:

Cryoem structure of pyocin r2 - precontracted - hub

Structure:

Probable bacteriophage protein pyocin r2. Chain: c, b, a

Source:

Pseudomonas aeruginosa (strain atcc 15692 / dsm 22644 / cip 104116 / jcm 14847 / lmg 12228 / 1c / prs 101 / pao1). Organism_taxid: 208964. Strain: atcc 15692 / dsm 22644 / cip 104116 / jcm 14847 / lmg 12228 / 1c / prs 101 / pao1

Authors:

P.Ge,J.Avaylon,D.Scholl,M.M.Shneider,C.Browning,S.A.Buth,M.Plattner, K.Ding,P.G.Leiman,J.F.Miller,Z.H.Zhou

Key ref:

P.Ge et al. (2020). Action of a minimal contractile bactericidal nanomachine. Nature, 580, 658-662. PubMed id: 32350467 DOI: 10.1038/s41586-020-2186-z

Date:

27-Aug-19 Release date: 15-Apr-20

Protein chains

G3XCU8  (G3XCU8_PSEAE) -  Phage late control D family protein from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

Seq: 329 a.a.

Struc: 318 a.a.

Enzyme reactions

• Enzyme class: E.C.?

DOI no: 10.1038/s41586-020-2186-z

Nature 580:658-662 (2020)

PubMed id: 32350467

Action of a minimal contractile bactericidal nanomachine.

P.Ge, D.Scholl, N.S.Prokhorov, J.Avaylon, M.M.Shneider, C.Browning, S.A.Buth, M.Plattner, U.Chakraborty, K.Ding, P.G.Leiman, J.F.Miller, Z.H.Zhou.

ABSTRACT

R-type bacteriocins are minimal contractile nanomachines that hold promise as precision antibiotics^1-4. Each bactericidal complex uses a collar to bridge a hollow tube with a contractile sheath loaded in a metastable state by a baseplate scaffold^1,2. Fine-tuning of such nucleic acid-free protein machines for precision medicine calls for an atomic description of the entire complex and contraction mechanism, which is not available from baseplate structures of the (DNA-containing) T4 bacteriophage⁵. Here we report the atomic model of the complete R2 pyocin in its pre-contraction and post-contraction states, each containing 384 subunits of 11 unique atomic models of 10 gene products. Comparison of these structures suggests the following sequence of events during pyocin contraction: tail fibres trigger lateral dissociation of baseplate triplexes; the dissociation then initiates a cascade of events leading to sheath contraction; and this contraction converts chemical energy into mechanical force to drive the iron-tipped tube across the bacterial cell surface, killing the bacterium.