PDBsum entry 6ts3

Peptide binding protein

PDB id: 6ts3

Contents

Protein chains

73 a.a.

20 a.a.

17 a.a.

Ligands

ACE ×2

Waters ×266

PDB id:

6ts3

Name:

Peptide binding protein

Title:

Ef-hands 3 and 4 of alpha-actinin in complex with camkii regulatory segment

Structure:

Alpha-actinin-2. Chain: a, b. Synonym: alpha-actinin skeletal muscle isoform 2,f-actin cross- linking protein. Engineered: yes. Ace-asn-ala-arg-arg-lys-leu-lys-gly-ala-ile-leu-thr-thr- met-leu-ala-thr-arg-asn-phe. Chain: c, d. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: actn2. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Mus musculus. House mouse.

Resolution:

1.28Å R-factor: 0.184 R-free: 0.199

Authors:

J.Zhu,M.Gold

Key ref:

J.Zhu and m.gold Ef-Hands 3 and 4 of alpha-Actinin in complex with cam regulatory segment. To be published, .

Date:

19-Dec-19 Release date: 13-Jan-21

Protein chains

P35609  (ACTN2_HUMAN) -  Alpha-actinin-2 from Homo sapiens

Seq: 894 a.a.

Struc: 73 a.a.*

Protein chain

P11798  (KCC2A_MOUSE) -  Calcium/calmodulin-dependent protein kinase type II subunit alpha from Mus musculus

Seq: 478 a.a.

Struc: 20 a.a.

Protein chain

P11798  (KCC2A_MOUSE) -  Calcium/calmodulin-dependent protein kinase type II subunit alpha from Mus musculus

Seq: 478 a.a.

Struc: 17 a.a.

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chains C, D: E.C.2.7.11.17 - calcium/calmodulin-dependent protein kinase.
• Reaction:
  1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
  2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺
• Cofactor: Ca(2+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site