PDBsum entry 6tjl

Transferase

PDB id: 6tjl

Contents

Protein chains

217 a.a.

Ligands

GSH ×2

Metals

_CA ×4

Waters ×118

PDB id:

6tjl

Name:

Transferase

Title:

Mature primitive pytochelatin synthase alr0975 from nostoc species bound to glutathione

Structure:

Alr0975 protein. Chain: a, b. Synonym: primitive phytochelatin synthase. Engineered: yes. Mutation: yes

Source:

Nostoc sp. Pcc 7120. Organism_taxid: 103690. Gene: alr0975, alr0975. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

1.87Å R-factor: 0.208 R-free: 0.268

Authors:

C.G.Feiler,W.Blankenfeldt

Key ref:

C.G.Feiler and w.blankenfeldt Mature primitive pytochelatin synthase alr0975 from n species bound to glutathione. To be published, .

Date:

26-Nov-19 Release date: 09-Dec-20

Protein chains

Q8YY76  (Q8YY76_NOSS1) -  glutathione gamma-glutamylcysteinyltransferase from Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576)

Seq: 242 a.a.

Struc: 217 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.2.3.2.15 - glutathione gamma-glutamylcysteinyltransferase.
• Reaction: [Glu(-Cys)](n)-Gly + glutathione + H⁺ = [Glu(-Cys)](n+1)-Gly + glycine

[Glu(-Cys)](n)-Gly (Bound ligand (Het Group name = GSH) corresponds exactly) + glutathione + H(+) = [Glu(-Cys)](n+1)-Gly + glycine

Molecule diagrams generated from .mol files obtained from the KEGG ftp site