PDBsum entry 6t5t

DNA binding protein

PDB id: 6t5t

Contents

Protein chain

404 a.a.

DNA/RNA

Ligands

BME ×6

Metals

_MG ×3

_CL

__K ×3

Waters ×318

PDB id:

6t5t

Name:

DNA binding protein

Title:

Crystal structure of archaeoglobus fulgidus argonaute protein with cognate DNA oligoduplex 5'-pattgtggccacaat

Structure:

Piwi protein af_1318. Chain: a. Fragment: arhaeoglobus fulgidus argonaute protein. Synonym: afago,afpiwi,piwi/mid domain protein. Engineered: yes. 5'-d( Ap Tp Tp Gp Tp Gp Gp Cp Cp Ap Cp Ap Ap Tp)-3'. Chain: r, s. Fragment: oligodeoxyribonucleotide. Engineered: yes

Source:

Archaeoglobus fulgidus (strain atcc 49558 / vc- 16 / dsm 4304 / jcm 9628 / nbrc 100126). Organism_taxid: 224325. Strain: atcc 49558 / vc-16 / dsm 4304 / jcm 9628 / nbrc 100126. Atcc: 49558. Gene: af_1318. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Synthetic: yes.

Resolution:

1.70Å R-factor: 0.181 R-free: 0.213

Authors:

S.Grazulis,M.Zaremba

Key ref:

E.Golovinas et al. Crystal structure of archaeoglobus fulgidus argonaute protein with cognate DNA oligoduplex 5'-Pattgtggccaca. To be published, .

Date:

17-Oct-19 Release date: 18-Nov-20

Protein chain

O28951  (PIWI_ARCFU) -  Piwi protein from Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16)

Seq: 427 a.a.

Struc: 404 a.a.

DNA/RNA chains

A-T-T-G-T-G-G-C-C-A-C-A-A-T 14 bases

A-T-T-G-T-G-G-C-C-A-C-A-A-T 14 bases