PDBsum entry 6t1f

DNA binding protein

PDB id: 6t1f

Contents

Protein chains

191 a.a.

DNA/RNA

PDB id:

6t1f

Name:

DNA binding protein

Title:

Crystal structure of thE C-terminally truncated chromosome- partitioning protein parb from caulobacter crescentus complexed to the centromeric pars site

Structure:

Chromosome-partitioning protein parb. Chain: a, b, c, d. Engineered: yes. Other_details: the expressed protein comprised residues 11-254 of the wild-type sequence with a c-terminal nickel affinity tag of sequence klaaalehhhhhh from the pet21b expression plasmid. DNA (5'- d( Gp Gp Ap Tp Gp Tp Tp Tp Cp Ap Cp Gp Tp Gp Ap Ap Ap Cp Ap Tp Cp C)- 3').

Source:

Caulobacter vibrioides (strain na1000 / cb15n). Organism_taxid: 565050. Strain: na1000 / cb15n. Gene: parb, ccna_03868. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Expression_system_variant: solu. Synthetic: yes. Caulobacter vibrioides.

Resolution:

2.90Å R-factor: 0.241 R-free: 0.263

Authors:

A.S.B.Jalal,C.L.Pastrana,N.T.Tran,C.E.M.Stevenson,D.M.Lawson, F.Moreno-Herrero,T.B.K.Le

Key ref:

A.S.B.Jalal et al. Structural and biochemical analyses of caulobacter crescentus parb reveal the role of its n-Terminal dom chromosome segregation. To be published, . PubMed id: 34397383

Date:

04-Oct-19 Release date: 14-Oct-20

Protein chains

B8GW30  (PARB_CAUVN) -  Chromosome-partitioning protein ParB from Caulobacter vibrioides (strain NA1000 / CB15N)

Seq: 304 a.a.

Struc: 191 a.a.

DNA/RNA chains

G-G-A-T-G-T-T-T-C-A-C-G-T-G-A-A-A-C-A-T-C-C 22 bases

G-G-A-T-G-T-T-T-C-A-C-G-T-G-A-A-A-C-A-T-C-C 22 bases

G-G-A-T-G-T-T-T-C-A-C-G-T-G-A-A-A-C-A-T-C-C 22 bases

G-G-A-T-G-T-T-T-C-A-C-G-T-G-A-A-A-C-A-T-C-C 22 bases

Enzyme reactions

• Enzyme class: E.C.?