PDBsum entry 6qw3

Structural protein

PDB id: 6qw3

Contents

Protein chain

110 a.a.

Metals

_CA

Waters ×150

PDB id:

6qw3

Name:

Structural protein

Title:

Calcium-bound gelsolin domain 2

Structure:

Gelsolin. Chain: a. Synonym: agel,actin-depolymerizing factor,adf,brevin. Engineered: yes. Other_details: gelsolin domain 2 (g2)

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: gsn. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

1.30Å R-factor: 0.156 R-free: 0.174

Authors:

E.Scalone,F.Boni,M.Milani,E.Mastrangelo,M.De Rosa

Key ref:

M.Bollati et al. (2019). High-resolution crystal structure of gelsolin domain 2 in complex with the physiological calcium ion. Biochem Biophys Res Commun, 518, 94-99. PubMed id: 31416615 DOI: 10.1016/j.bbrc.2019.08.013

Date:

05-Mar-19 Release date: 28-Aug-19

Protein chain

P06396  (GELS_HUMAN) -  Gelsolin from Homo sapiens

Seq: 782 a.a.

Struc: 110 a.a.

DOI no: 10.1016/j.bbrc.2019.08.013

Biochem Biophys Res Commun 518:94-99 (2019)

PubMed id: 31416615

High-resolution crystal structure of gelsolin domain 2 in complex with the physiological calcium ion.

M.Bollati, E.Scalone, F.Bonì, E.Mastrangelo, T.Giorgino, M.Milani, M.de Rosa.

ABSTRACT

The second domain of gelsolin (G2) hosts mutations responsible for a hereditary form of amyloidosis. The active form of gelsolin is Ca²⁺-bound; it is also a dynamic protein, hence structural biologists often rely on the study of the isolated G2. However, the wild type G2 structure that have been used so far in comparative studies is bound to a crystallographic Cd²⁺, in lieu of the physiological calcium. Here, we report the wild type structure of G2 in complex with Ca²⁺ highlighting subtle ion-dependent differences. Previous findings on different G2 mutations are also briefly revised in light of these results.