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PDBsum entry 6pr0
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PDB id:
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Transferase
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Title:
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P133h-s128a s. Typhimurium siroheme synthase
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Structure:
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Siroheme synthase. Chain: a, b. Engineered: yes. Mutation: yes
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Source:
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Salmonella typhimurium. Organism_taxid: 90371. Gene: cysg, coba, aap89_23165, abo94_22995, af480_23265, af488_22710, af489_21700, aic76_23675, axr84_23260, axu58_22185, c2253_19735, cd48_22680, ce87_23070, cet98_25025, cvr97_13625, d7f20_23535, d7h43_21790, dj388_17225, e2f01_22980, fcj89_03505, fga22_01875, fga24_01980, fga25_01865, fga26_01870, gw08_22845, jo10_22525, lz63_24065, nctc13348_03825, ng18_22490, nu83_22495, qa89_22165, qb40_24005, qd15_23475, rj78_23420, se14_03689,
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Resolution:
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1.90Å
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R-factor:
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0.181
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R-free:
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0.229
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Authors:
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J.M.Pennington,M.E.Stroupe
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Date:
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10-Jul-19
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Release date:
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26-Feb-20
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PROCHECK
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Headers
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References
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P25924
(CYSG_SALTY) -
Siroheme synthase from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
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Seq:
Struc:
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457 a.a.
451 a.a.*
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Key: |
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PfamA domain |
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Secondary structure |
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*
PDB and UniProt seqs differ
at 2 residue positions (black
crosses)
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Enzyme class 1:
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E.C.1.3.1.76
- precorrin-2 dehydrogenase.
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Pathway:
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Corrin and Siroheme Biosynthesis (part 2)
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Reaction:
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precorrin-2 + NAD+ = sirohydrochlorin + NADH + 2 H+
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precorrin-2
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+
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NAD(+)
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=
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sirohydrochlorin
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+
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NADH
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+
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2
×
H(+)
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Enzyme class 2:
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E.C.2.1.1.107
- uroporphyrinogen-III C-methyltransferase.
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Pathway:
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Reaction:
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uroporphyrinogen III + 2 S-adenosyl-L-methionine = precorrin-2 + 2 S-adenosyl-L-homocysteine + H+
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uroporphyrinogen III
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+
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2
×
S-adenosyl-L-methionine
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=
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precorrin-2
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+
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2
×
S-adenosyl-L-homocysteine
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+
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2
×
H(+)
Bound ligand (Het Group name = )
corresponds exactly
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Enzyme class 3:
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E.C.4.99.1.4
- sirohydrochlorin ferrochelatase.
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Pathway:
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Reaction:
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siroheme + 2 H+ = sirohydrochlorin + Fe2+
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siroheme
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+
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2
×
H(+)
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=
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sirohydrochlorin
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+
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2
×
Fe(2+)
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Note, where more than one E.C. class is given (as above), each may
correspond to a different protein domain or, in the case of polyprotein
precursors, to a different mature protein.
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Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
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Links
