PDBsum entry 6p3c

Lyase

PDB id: 6p3c

Contents

Protein chains

461 a.a.

Ligands

FLC ×2

Waters ×862

PDB id:

6p3c

Name:

Lyase

Title:

E. Coli fumarase mutant - t187a

Structure:

Fumarate hydratase class ii. Chain: a, b. Synonym: fumarasE C,aerobic fumarase,iron-independent fumarase. Engineered: yes. Mutation: yes

Source:

Escherichia coli (strain k12). Organism_taxid: 83333. Strain: k12. Gene: fumc, b1611, jw1603. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

1.46Å R-factor: 0.163 R-free: 0.182

Authors:

J.F.May,B.Bhattcharyya,T.M.Weaver

Key ref:

J.F.May et al. Fumarase c variant at the active site. To be published, .

Date:

23-May-19 Release date: 27-May-20

Protein chains

P05042  (FUMC_ECOLI) -  Fumarate hydratase class II from Escherichia coli (strain K12)

Seq: 467 a.a.

Struc: 461 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.4.2.1.2 - fumarate hydratase.
• Pathway: Citric acid cycle
• Reaction: (S)-malate = fumarate + H2O

(S)-malate (Bound ligand (Het Group name = FLC) matches with 69.23% similarity) = fumarate + H2O

Molecule diagrams generated from .mol files obtained from the KEGG ftp site