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PDBsum entry 6omc
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PDB id:
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Virus
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Title:
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Capsid of t5 virion
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Structure:
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Major capsid protein. Chain: a, b, c, d, e, f, g, h, i, j, k, l, m. Synonym: capsid protein pb8, major head protein
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Source:
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Escherichia phage t5. Organism_taxid: 10726
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Authors:
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A.Huet,R.L.Duda,P.Boulanger,J.F.Conway
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Key ref:
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A.Huet
et al.
(2019).
Capsid expansion of bacteriophage T5 revealed by high resolution cryoelectron microscopy.
Proc Natl Acad Sci U S A,
116,
21037-21046.
PubMed id:
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Date:
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18-Apr-19
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Release date:
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02-Oct-19
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PROCHECK
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Headers
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References
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Q6QGD8
(CAPSD_BPT5) -
Major capsid protein from Escherichia phage T5
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Seq:
Struc:
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458 a.a.
299 a.a.
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Key: |
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PfamA domain |
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Secondary structure |
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Proc Natl Acad Sci U S A
116:21037-21046
(2019)
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PubMed id:
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Capsid expansion of bacteriophage T5 revealed by high resolution cryoelectron microscopy.
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A.Huet,
R.L.Duda,
P.Boulanger,
J.F.Conway.
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ABSTRACT
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The large (90-nm) icosahedral capsid of bacteriophage T5 is composed of 775
copies of the major capsid protein (mcp) together with portal, protease, and
decoration proteins. Its assembly is a regulated process that involves several
intermediates, including a thick-walled round precursor prohead that expands as
the viral DNA is packaged to yield a thin-walled and angular mature capsid. We
investigated capsid maturation by comparing cryoelectron microscopy (cryo-EM)
structures of the prohead, the empty expanded capsid both with and without
decoration protein, and the virion capsid at a resolution of 3.8 Å for the
latter. We detail the molecular structure of the mcp, its complex pattern of
interactions, and their evolution during maturation. The bacteriophage T5 mcp is
a variant of the canonical HK97-fold with a high level of plasticity that allows
for the precise assembly of a giant macromolecule and the adaptability needed to
interact with other proteins and the packaged DNA.
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Links
