PDBsum entry 6mls

Lyase

PDB id: 6mls

Contents

Protein chains

455 a.a.

Ligands

CQG ×3

0JO

P33

Metals

__K ×2

Waters ×619

PDB id:

6mls

Name:

Lyase

Title:

Citrobacter freundii tyrosine phenol-lyase complexed with 4- hydroxypyridine and aminoacrylate from l-tyrosine

Structure:

Tyrosine phenol-lyase. Chain: a. Synonym: beta-tyrosinase. Engineered: yes. Tyrosine phenol-lyase. Chain: b. Synonym: beta-tyrosinase. Engineered: yes

Source:

Citrobacter freundii. Organism_taxid: 546. Atcc: 29063. Gene: tpl. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

1.77Å R-factor: 0.167 R-free: 0.193

Authors:

R.S.Phillips

Key ref:

R.S.Phillips Structure of aminoacrylate intermediates of tyrosine phenol-Lyase. To be published, .

Date:

27-Sep-18 Release date: 02-Oct-19

Protein chains

P31013  (TPL_CITFR) -  Tyrosine phenol-lyase from Citrobacter freundii

Seq: 456 a.a.

Struc: 455 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.4.1.99.2 - tyrosine phenol-lyase.
• Reaction: L-tyrosine + H2O = phenol + pyruvate + NH4⁺

L-tyrosine + H2O = phenol + pyruvate (Bound ligand (Het Group name = CQG) matches with 75.00% similarity) + NH4(+)

• Cofactor: Pyridoxal 5'-phosphate

Pyridoxal 5'-phosphate (Bound ligand (Het Group name = 0JO) matches with 68.18% similarity)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site