PDBsum entry 6m2c

Transferase

PDB id: 6m2c

Contents

Protein chains

149 a.a.

55 a.a.

Metals

_ZN ×8

Waters ×62

PDB id:

6m2c

Name:

Transferase

Title:

Distinct mechanism of mul1-ring domain simultaneously recruiting e2 enzyme and the substrate p53-tad domain

Structure:

Ubiquitin-conjugating enzyme e2 d2. Chain: a, b, c, d. Synonym: (e3-independent) e2 ubiquitin-conjugating enzyme d2,e2 ubiquitin-conjugating enzyme d2,ubiquitin carrier protein d2, ubiquitin-conjugating enzyme e2(17)kb 2,ubiquitin-conjugating enzyme e2-17 kda 2,ubiquitin-protein ligase d2,p53-regulated ubiquitin- conjugating enzyme 1. Engineered: yes. Mitochondrial ubiquitin ligase activator of nfkb 1.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: ube2d2, pubc1, ubc4, ubc5b, ubch4, ubch5b. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: mul1, c1orf166, gide, mapl, mulan, rnf218. Expression_system_taxid: 562

Resolution:

2.70Å R-factor: 0.216 R-free: 0.258

Authors:

S.O.Lee,K.S.Ryu,S.-W.Chi

Key ref:

S.O.Lee et al. Distinct mechanism of mul1-Ring domain simultaneously recruiting e2 enzyme and the substrate p53-Tad domain. To be published, . PubMed id: 35048531

Date:

27-Feb-20 Release date: 07-Apr-21

Protein chains

P62837  (UB2D2_HUMAN) -  Ubiquitin-conjugating enzyme E2 D2 from Homo sapiens

Seq: 147 a.a.

Struc: 149 a.a.

Protein chains

Q969V5  (MUL1_HUMAN) -  Mitochondrial ubiquitin ligase activator of NFKB 1 from Homo sapiens

Seq: 352 a.a.

Struc: 55 a.a.

Enzyme reactions

• Enzyme class 1: Chains A, B, C, D: E.C.2.3.2.23 - E2 ubiquitin-conjugating enzyme.
• Reaction: S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L- cysteine
• Enzyme class 2: Chains A, B, C, D: E.C.2.3.2.24 - (E3-independent) E2 ubiquitin-conjugating enzyme.
• Reaction: S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + N₆- monoubiquitinyl-[acceptor protein]-L-lysine

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.