PDBsum entry 6lwc

DNA binding protein/DNA

PDB id: 6lwc

Contents

Protein chains

265 a.a.

DNA/RNA

Waters ×55

PDB id:

6lwc

Name:

DNA binding protein/DNA

Title:

Crystal structure of human neil1(p2g, e3q, k242) bound to duplex DNA containing spiroiminodihydantoin (sp)

Structure:

Endonuclease 8-like 1. Chain: a, d. Synonym: DNA glycosylase/ap lyase neil1,DNA-(apurinic or apyrimidinic site) lyase neil1,endonuclease viii-like 1,fpg1,nei homolog 1,neh1, nei-like protein 1. Engineered: yes. Mutation: yes. DNA (5'-d( Cp Gp Tp Cp Cp Ap (Dsp)p Gp Tp Cp Tp Ap C)-3'). Chain: b, e.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: neil1. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Escherichia coli. Organism_taxid: 562.

Resolution:

2.91Å R-factor: 0.193 R-free: 0.243

Authors:

M.H.Liu,J.Zhang,C.X.Zhu,X.X.Zhang,Y.Q.Gao,C.Q.Yi

Key ref:

M.Liu et al. Dna repair glycosylase hneil1 triages damaged bases V competing interaction modes.. To be published, . PubMed id: 34226550

Date:

07-Feb-20 Release date: 09-Jun-21

Protein chains

Q96FI4  (NEIL1_HUMAN) -  Endonuclease 8-like 1 from Homo sapiens

Seq: 390 a.a.

Struc: 265 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

DNA/RNA chains

C-G-T-C-C-A-EWC-G-T-C-T-A-C 13 bases

T-A-G-A-C-C-T-G-G-A-C-G-G 13 bases

C-G-T-C-C-A-EWC-G-T-C-T-A-C 13 bases

T-A-G-A-C-C-T-G-G-A-C-G-G 13 bases

Enzyme reactions

• Enzyme class 1: E.C.3.2.2.- - ?????
• Enzyme class 2: E.C.4.2.99.18 - DNA-(apurinic or apyrimidinic site) lyase.
• Reaction: 2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)- 2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3- dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-phospho- 2'-deoxyribonucleoside-DNA + H⁺

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.