PDBsum entry 6lhs

DNA repair

PDB id: 6lhs

Contents

Protein chains

719 a.a.

PDB id:

6lhs

Name:

DNA repair

Title:

High resolution structure of fanca c-terminal domain (ctd)

Structure:

Fanconi anemia complementation group a. Chain: b, a. Engineered: yes

Source:

Xenopus laevis. African clawed frog. Organism_taxid: 8355. Gene: fanca, fanca. Expressed in: trichoplusia ni. Expression_system_taxid: 7111

Authors:

E.Jeong,S.Lee,J.Shin,Y.Kim,O.Scharer,Y.Kim,H.Kim,Y.Cho

Key ref:

E.Jeong et al. (2020). Structural basis of the fanconi anemia-associated mutations within the FANCA and FANCG complex. Nucleic Acids Res, 48, 3328-3342. PubMed id: 32002546 DOI: 10.1093/nar/gkaa062

Date:

10-Dec-19 Release date: 25-Mar-20

Protein chains

Q4VT51  (Q4VT51_XENLA) -  FA complementation group A L homeolog from Xenopus laevis

Seq: 1422 a.a.

Struc: 719 a.a.

Enzyme reactions

• Enzyme class: E.C.?

DOI no: 10.1093/nar/gkaa062

Nucleic Acids Res 48:3328-3342 (2020)

PubMed id: 32002546

Structural basis of the fanconi anemia-associated mutations within the FANCA and FANCG complex.

E.Jeong, S.G.Lee, H.S.Kim, J.Yang, J.Shin, Y.Kim, J.Kim, O.D.Schärer, Y.Kim, J.E.Yeo, H.M.Kim, Y.Cho.

ABSTRACT

Monoubiquitination of the Fanconi anemia complementation group D2 (FANCD2) protein by the FA core ubiquitin ligase complex is the central event in the FA pathway. FANCA and FANCG play major roles in the nuclear localization of the FA core complex. Mutations of these two genes are the most frequently observed genetic alterations in FA patients, and most point mutations in FANCA are clustered in the C-terminal domain (CTD). To understand the basis of the FA-associated FANCA mutations, we determined the cryo-electron microscopy (EM) structures of Xenopus laevis FANCA alone at 3.35 Å and 3.46 Å resolution and two distinct FANCA-FANCG complexes at 4.59 and 4.84 Å resolution, respectively. The FANCA CTD adopts an arc-shaped solenoid structure that forms a pseudo-symmetric dimer through its outer surface. FA- and cancer-associated point mutations are widely distributed over the CTD. The two different complex structures capture independent interactions of FANCG with either FANCA C-terminal HEAT repeats, or the N-terminal region. We show that mutations that disturb either of these two interactions prevent the nuclear localization of FANCA, thereby leading to an FA pathway defect. The structure provides insights into the function of FANCA CTD, and provides a framework for understanding FA- and cancer-associated mutations.