PDBsum entry 6gx2

Transferase

PDB id: 6gx2

Contents

Protein chain

297 a.a.

Ligands

UDP

GOL ×2

EDO ×3

SO4 ×2

Metals

_MN

Waters ×457

PDB id:

6gx2

Name:

Transferase

Title:

Blood group synthase aaglyb in complex with udp-galnac and cryoprotected with glycerol

Structure:

Abo blood group (transferase a, alpha 1-3-n- acetylgalactosaminyltransferase transferase b, alpha 1-3- galactosyltransferase). Chain: a. Engineered: yes. Mutation: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: abo. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

1.07Å R-factor: 0.113 R-free: 0.134

Authors:

J.Rocha,A.Royant

Key ref:

J.Rocha et al. Blood group synthase aaglyb in complex with udp-Galna cryoprotected with glycerol. To be published, .

Date:

26-Jun-18 Release date: 10-Jul-19

Protein chain

P16442  (BGAT_HUMAN) -  Histo-blood group ABO system transferase from Homo sapiens

Seq: 354 a.a.

Struc: 297 a.a.*

* PDB and UniProt seqs differ at 8 residue positions (black crosses)

Enzyme reactions

• Enzyme class 1: E.C.2.4.1.37 - fucosylgalactoside 3-alpha-galactosyltransferase.
• Reaction: an alpha-L-fucosyl-(1->2)-beta-D-galactosyl derivative + UDP-alpha-D- galactose = an alpha-D-galactosyl-(1->3)-[alpha-L-fucosyl-(1->2)]-beta-D- galactosyl derivative + UDP + H⁺

alpha-L-fucosyl-(1->2)-beta-D-galactosyl derivative + UDP-alpha-D- galactose = alpha-D-galactosyl-(1->3)-[alpha-L-fucosyl-(1->2)]-beta-D- galactosyl derivative + UDP + H(+) (Bound ligand (Het Group name = UDP) corresponds exactly)

• Enzyme class 2: E.C.2.4.1.40 - glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase.
• Reaction: an alpha-L-fucosyl-(1->2)-beta-D-galactosyl derivative + UDP-N-acetyl- alpha-D-galactosamine = an N-acetyl-alpha-D-galactosaminyl-(1->3)-[alpha- L-fucosyl-(1->2)]-beta-D-galactosyl derivative + UDP + H⁺

alpha-L-fucosyl-(1->2)-beta-D-galactosyl derivative + UDP-N-acetyl- alpha-D-galactosamine = N-acetyl-alpha-D-galactosaminyl-(1->3)-[alpha- L-fucosyl-(1->2)]-beta-D-galactosyl derivative + UDP + H(+) (Bound ligand (Het Group name = UDP) corresponds exactly)

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site