PDBsum entry 6f8s

Toxin

PDB id: 6f8s

Contents

Protein chains

94 a.a.

66 a.a.

65 a.a.

Ligands

SO4 ×2

PEG ×3

Waters ×11

PDB id:

6f8s

Name:

Toxin

Title:

Toxin-antitoxin complex grata

Structure:

Xre family transcriptional regulator. Chain: a, c. Engineered: yes. Other_details: antitoxin graa. Putative killer protein. Chain: b, d. Engineered: yes. Other_details: toxin grat

Source:

Pseudomonas putida. Arthrobacter siderocapsulatus. Organism_taxid: 303. Gene: ayo08_18510. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Pseudomonas putida (strain atcc 47054 / dsm 6125 / ncimb 11950 / kt2440). Organism_taxid: 160488.

Resolution:

2.50Å R-factor: 0.180 R-free: 0.212

Authors:

A.Talavera,R.Loris

Key ref:

A.Talavera et al. (2019). A dual role in regulation and toxicity for the disordered N-terminus of the toxin GraT. Nat Commun, 10, 972. PubMed id: 30814507 DOI: 10.1038/s41467-019-08865-z

Date:

13-Dec-17 Release date: 11-Jul-18

Protein chains

Q88MI6  (Q88MI6_PSEPK) -  Antidote protein from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440)

Seq: 99 a.a.

Struc: 94 a.a.

Protein chain

Q88MI5  (Q88MI5_PSEPK) -  Killer protein from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440)

Seq: 92 a.a.

Struc: 66 a.a.

Protein chain

Q88MI5  (Q88MI5_PSEPK) -  Killer protein from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440)

Seq: 92 a.a.

Struc: 65 a.a.

Enzyme reactions

• Enzyme class: Chains A, B, C, D: E.C.?

DOI no: 10.1038/s41467-019-08865-z

Nat Commun 10:972 (2019)

PubMed id: 30814507

A dual role in regulation and toxicity for the disordered N-terminus of the toxin GraT.

A.Talavera, H.Tamman, A.Ainelo, A.Konijnenberg, S.Hadži, F.Sobott, A.Garcia-Pino, R.Hõrak, R.Loris.

ABSTRACT

Bacterial toxin-antitoxin (TA) modules are tightly regulated to maintain growth in favorable conditions or growth arrest during stress. A typical regulatory strategy involves the antitoxin binding and repressing its own promoter while the toxin often acts as a co-repressor. Here we show that Pseudomonas putida graTA-encoded antitoxin GraA and toxin GraT differ from other TA proteins in the sense that not the antitoxin but the toxin possesses a flexible region. GraA auto-represses the graTA promoter: two GraA dimers bind cooperatively at opposite sides of the operator sequence. Contrary to other TA modules, GraT is a de-repressor of the graTA promoter as its N-terminal disordered segment prevents the binding of the GraT₂A₂ complex to the operator. Removal of this region restores operator binding and abrogates Gr aT toxicity. GraTA represents a TA module where a flexible region in the toxin rather than in the antitoxin controls operon expression and toxin activity.