PDBsum entry 6f5e

De novo protein

PDB id: 6f5e

Contents

Protein chains

315 a.a.

346 a.a.

Ligands

PRO-LYS-ARG-PRO-THR-THR-LEU-ASN-LEU-PHE

PDB id:

6f5e

Name:

De novo protein

Title:

Crystal structure of darpin-darpin rigid fusion, variant dd_d12_10_47 in complex jnk1a1 and jip1 peptide

Structure:

Dd_d12_10_47. Chain: a. Engineered: yes. Mitogen-activated protein kinase 8. Chain: b. Synonym: mapk 8,jnk-46,stress-activated protein kinase 1c,sapk1c, stress-activated protein kinase jnk1,c-jun n-terminal kinase 1. Engineered: yes. C-jun-amino-terminal kinase-interacting protein 1.

Source:

Synthetic construct. Organism_taxid: 32630. Expressed in: escherichia coli k-12. Expression_system_taxid: 83333. Expression_system_variant: xl1-blue. Homo sapiens. Human. Organism_taxid: 9606. Gene: mapk8, jnk1, prkm8, sapk1, sapk1c.

Resolution:

2.70Å R-factor: 0.202 R-free: 0.226

Authors:

Y.Wu,P.R.Mittl,A.Honegger,A.Batyuk,A.Plueckthun

Key ref:

Y.Wu et al. Crystal structure of darpin-Darpin rigid fusion, Vari dd_d12_10_47 in complex jnk1a1 and jip1 peptide. To be published, .

Date:

01-Dec-17 Release date: 13-Dec-17

Supersedes:

5len

Protein chain

No UniProt id for this chain

Struc: 315 a.a.

Protein chain

P45983  (MK08_HUMAN) -  Mitogen-activated protein kinase 8 from Homo sapiens

Seq: 427 a.a.

Struc: 346 a.a.

Enzyme reactions

• Enzyme class: Chain B: E.C.2.7.11.24 - mitogen-activated protein kinase.
• Reaction:
  1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
  2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site