PDBsum entry 6d0t

De novo protein

PDB id: 6d0t

Contents

Protein chains

111 a.a.

Waters ×106

PDB id:

6d0t

Name:

De novo protein

Title:

De novo design of a fluorescence-activating beta barrel - bb1

Structure:

Bb1. Chain: a, b. Engineered: yes

Source:

Synthetic construct. Organism_taxid: 32630. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

1.63Å R-factor: 0.153 R-free: 0.184

Authors:

J.Dou,A.A.Vorobieva,W.Sheffler,L.A.Doyle,H.Park,M.J.Bick,B.Mao, G.W.Foight,M.Lee,L.Carter,B.Sankaran,S.Ovchinnikov,E.Marcos,P.Huang, J.C.Vaughan,B.L.Stoddard,D.Baker

Key ref:

J.Dou et al. (2018). De novo design of a fluorescence-activating β-barrel. Nature, 561, 485-491. PubMed id: 30209393 DOI: 10.1038/s41586-018-0509-0

Date:

10-Apr-18 Release date: 19-Sep-18

Protein chains

No UniProt id for this chain

Struc: 111 a.a.

DOI no: 10.1038/s41586-018-0509-0

Nature 561:485-491 (2018)

PubMed id: 30209393

De novo design of a fluorescence-activating β-barrel.

J.Dou, A.A.Vorobieva, W.Sheffler, L.A.Doyle, H.Park, M.J.Bick, B.Mao, G.W.Foight, M.Y.Lee, L.A.Gagnon, L.Carter, B.Sankaran, S.Ovchinnikov, E.Marcos, P.S.Huang, J.C.Vaughan, B.L.Stoddard, D.Baker.

ABSTRACT

The regular arrangements of β-strands around a central axis in β-barrels and of α-helices in coiled coils contrast with the irregular tertiary structures of most globular proteins, and have fascinated structural biologists since they were first discovered. Simple parametric models have been used to design a wide range of α-helical coiled-coil structures, but to date there has been no success with β-barrels. Here we show that accurate de novo design of β-barrels requires considerable symmetry-breaking to achieve continuous hydrogen-bond connectivity and eliminate backbone strain. We then build ensembles of β-barrel backbone models with cavity shapes that match the fluorogenic compound DFHBI, and use a hierarchical grid-based search method to simultaneously optimize the rigid-body placement of DFHBI in these cavities and the identities of the surrounding amino acids to achieve high shape and chemical complementarity. The designs have high structural accuracy and bind and fluorescently activate DFHBI in vitro and in Escherichia coli, yeast and mammalian cells. This de novo design of small-molecule binding activity, using backbones custom-built to bind the ligand, should enable the design of increasingly sophisticated ligand-binding proteins, sensors and catalysts that are not limited by the backbone geometries available in known protein structures.