PDBsum entry 6blk

Transferase

PDB id: 6blk

Contents

Protein chains

158 a.a.

149 a.a.

Ligands

ATP ×4

Metals

_MG ×4

Waters ×586

PDB id:

6blk

Name:

Transferase

Title:

Mycobacterial sensor histidine kinase mprb

Structure:

Signal transduction histidine-protein kinase/phosphatase mprb. Chain: c, d, a, b. Engineered: yes. Other_details: fragment residues 322-476, n-terminal gam residues are from vector, note that uniprot entry has a mis-annotated start codon - therefore a difference in numbering

Source:

Mycobacterium hassiacum (strain dsm 44199 / cip 105218 / jcm 12690 / 3849). Organism_taxid: 1122247. Strain: dsm 44199 / cip 105218 / jcm 12690 / 3849. Gene: mprb, c731_3480. Expressed in: escherichia coli. Expression_system_taxid: 511693.

Resolution:

1.55Å R-factor: 0.186 R-free: 0.221

Authors:

J.Li,N.Korotkova,K.V.Korotkov

Key ref:

J.Li et al. Mycobacterial sensor histidine kinase mprb. To be published, .

Date:

10-Nov-17 Release date: 22-Nov-17

Protein chains

K5BDW2  (K5BDW2_MYCHD) -  histidine kinase from Mycolicibacterium hassiacum (strain DSM 44199 / CIP 105218 / JCM 12690 / 3849)

Seq: 466 a.a.

Struc: 158 a.a.*

Protein chains

K5BDW2  (K5BDW2_MYCHD) -  histidine kinase from Mycolicibacterium hassiacum (strain DSM 44199 / CIP 105218 / JCM 12690 / 3849)

Seq: 466 a.a.

Struc: 149 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: Chains C, D, A, B: E.C.2.7.13.3 - histidine kinase.
• Reaction: ATP + protein L-histidine = ADP + protein N-phospho-L-histidine

ATP (Bound ligand (Het Group name = ATP) corresponds exactly) + protein L-histidine = ADP + protein N-phospho-L-histidine

Molecule diagrams generated from .mol files obtained from the KEGG ftp site