PDBsum entry 6bbm

Replication

PDB id: 6bbm

Contents

Protein chains

405 a.a.

447 a.a.

125 a.a.

Ligands

ADP ×5

PDB id:

6bbm

Name:

Replication

Title:

Mechanisms of opening and closing of the bacterial replicative helicase: the dnab helicase and lambda p helicase loader complex

Structure:

Replicative DNA helicase. Chain: a, b, c, d, e, f. Engineered: yes. Replication protein p. Chain: v, w, x, y, z. Engineered: yes

Source:

Escherichia coli o111:nm. Organism_taxid: 373045. Gene: dqw49_15020. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Expression_system_cell_line: bl21(de3). Escherichia phage lambda. Organism_taxid: 10710.

Authors:

J.Chase,A.Catalano,A.J.Noble,E.T.Eng,P.D.B.Olinares,K.Molloy, D.Pakotiprapha,M.Samuels,B.Chain,A.Des Georges,D.Jeruzalmi

Key ref:

J.Chase et al. (2018). Mechanisms of opening and closing of the bacterial replicative helicase.. Elife, 7, . PubMed id: 30582519

Date:

18-Oct-17 Release date: 06-Mar-19

Protein chain

P0ACB0  (DNAB_ECOLI) -  Replicative DNA helicase from Escherichia coli (strain K12)

Seq: 471 a.a.

Struc: 405 a.a.

Protein chains

P0ACB0  (DNAB_ECOLI) -  Replicative DNA helicase from Escherichia coli (strain K12)

Seq: 471 a.a.

Struc: 447 a.a.

Protein chains

P03689  (VRPP_LAMBD) -  Replication protein P from Escherichia phage lambda

Seq: 233 a.a.

Struc: 125 a.a.

Enzyme reactions

• Enzyme class 2: Chains A, B, C, D, E, F: E.C.3.6.4.12 - Dna helicase.
• Reaction: ATP + H2O = ADP + phosphate + H⁺

ATP + H2O = ADP (Bound ligand (Het Group name = ADP) corresponds exactly) + phosphate + H(+)

• Enzyme class 3: Chains V, W, X, Y, Z: E.C.?

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site