PDBsum entry 6b8f

Oxidoreductase

PDB id: 6b8f

Contents

Protein chain

172 a.a.

Metals

_CA ×11

_FE ×2

Waters ×340

PDB id:

6b8f

Name:

Oxidoreductase

Title:

Contracted human heavy-chain ferritin crystal-hydrogel hybrid

Structure:

Ferritin heavy chain. Chain: a. Synonym: ferritin h subunit,cell proliferation-inducing gene 15 protein. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: fth1, fth, fthl6, ok/sw-cl.84, pig15. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

1.06Å R-factor: 0.092 R-free: 0.103

Authors:

L.Zhang,J.B.Bailey,R.Subramanian,F.A.Tezcan

Key ref:

L.Zhang et al. (2018). Hyperexpandable, self-healing macromolecular crystals with integrated polymer networks. Nature, 557, 86-91. PubMed id: 29720635 DOI: 10.1038/s41586-018-0057-7

Date:

07-Oct-17 Release date: 02-May-18

Protein chain

P02794  (FRIH_HUMAN) -  Ferritin heavy chain from Homo sapiens

Seq: 183 a.a.

Struc: 172 a.a.*

* PDB and UniProt seqs differ at 4 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.1.16.3.1 - ferroxidase.
• Reaction: 4 Fe²⁺ + O2 + 4 H⁺ = 4 Fe³⁺ + 2 H2O
• Cofactor: Cu cation

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Key reference

DOI no: 10.1038/s41586-018-0057-7

Nature 557:86-91 (2018)

PubMed id: 29720635

Hyperexpandable, self-healing macromolecular crystals with integrated polymer networks.

L.Zhang, J.B.Bailey, R.H.Subramanian, F.A.Tezcan.

ABSTRACT

The formation of condensed matter typically involves a trade-off between structural order and flexibility. As the extent and directionality of interactions between atomic or molecular components increase, materials generally become more ordered but less compliant, and vice versa. Nevertheless, high levels of structural order and flexibility are not necessarily mutually exclusive; there are many biological (such as microtubules^1,2, flagella ³ , viruses^4,5) and synthetic assemblies (for example, dynamic molecular crystals^6-9 and frameworks^10-13) that can undergo considerable structural transformations without losing their crystalline order and that have remarkable mechanical properties^8,14,15 that are useful in diverse applications, such as selective sorption ¹⁶ , separation ¹⁷ , sensing ¹⁸ and mechanoactuation ¹⁹ . However, the extent of structural changes and the elasticity of such flexible crystals are constrained by the necessity to maintain a continuous network of bonding interactions between the constituents of the lattice. Consequently, even the most dynamic porous materials tend to be brittle and isolated as microcrystalline powders ¹⁴ , whereas flexible organic or inorganic molecular crystals cannot expand without fracturing. Owing to their rigidity, crystalline materials rarely display self-healing behaviour ²⁰ . Here we report that macromolecular ferritin crystals with integrated hydrogel polymers can isotropically expand to 180 per cent of their original dimensions and more than 500 per cent of their original volume while retaining periodic order and faceted Wulff morphologies. Even after the separation of neighbouring ferritin molecules by 50 ångströms upon lattice expansion, specific molecular contacts between them can be reformed upon lattice contraction, resulting in the recovery of atomic-level periodicity and the highest-resolution ferritin structure reported so far. Dynamic bonding interactions between the hydrogel network and the ferritin molecules endow the crystals with the ability to resist fragmentation and self-heal efficiently, whereas the chemical tailorability of the ferritin molecules enables the creation of chemically and mechanically differentiated domains within single crystals.