PDBsum entry 6b6b

Hydrolase

PDB id: 6b6b

Contents

Protein chain

265 a.a.

Waters ×167

PDB id:

6b6b

Name:

Hydrolase

Title:

Beta-lactamase, unmixed needles crystal form

Structure:

Beta-lactamase. Chain: a. Synonym: ambler class a beta-lactamase. Engineered: yes

Source:

Mycobacterium tuberculosis (strain atcc 25618 / h37rv). Organism_taxid: 83332. Strain: atcc 25618 / h37rv. Gene: blac, blaa, rv2068c, mtcy49.07c. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

1.80Å R-factor: 0.217 R-free: 0.245

Authors:

S.Pandey,M.Schmidt

Key ref:

J.L.Olmos et al. (2018). Enzyme intermediates captured "on the fly" by mix-and-inject serial crystallography. BMC Biol, 16, 59. PubMed id: 29848358

Date:

01-Oct-17 Release date: 27-Jun-18

Protein chain

P9WKD3  (BLAC_MYCTU) -  Beta-lactamase from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

Seq: 307 a.a.

Struc: 265 a.a.

Enzyme reactions

• Enzyme class: E.C.3.5.2.6 - beta-lactamase.
• Pathway: Penicillin Biosynthesis and Metabolism
• Reaction: a beta-lactam + H2O = a substituted beta-amino acid
• Cofactor: Zn(2+)

BMC Biol 16:59 (2018)

PubMed id: 29848358

Enzyme intermediates captured "on the fly" by mix-and-inject serial crystallography.

J.L.Olmos, S.Pandey, J.M.Martin-Garcia, G.Calvey, A.Katz, J.Knoska, C.Kupitz, M.S.Hunter, M.Liang, D.Oberthuer, O.Yefanov, M.Wiedorn, M.Heyman, M.Holl, K.Pande, A.Barty, M.D.Miller, S.Stern, S.Roy-Chowdhury, J.Coe, N.Nagaratnam, J.Zook, J.Verburgt, T.Norwood, I.Poudyal, D.Xu, J.Koglin, M.H.Seaberg, Y.Zhao, S.Bajt, T.Grant, V.Mariani, G.Nelson, G.Subramanian, E.Bae, R.Fromme, R.Fung, P.Schwander, M.Frank, T.A.White, U.Weierstall, N.Zatsepin, J.Spence, P.Fromme, H.N.Chapman, L.Pollack, L.Tremblay, A.Ourmazd, G.N.Phillips, M.Schmidt.

ABSTRACT

No abstract given.