PDBsum entry 6afp

Hydrolase

PDB id: 6afp

Contents

Protein chains

268 a.a.

Ligands

CB4 ×2

ACT

Waters ×611

PDB id:

6afp

Name:

Hydrolase

Title:

Crystal structure of class a b-lactamase, penl, variant asn136asp, from burkholderia thailandensis, in complex with ceftazidime-like boronic acid

Structure:

Beta-lactamase. Chain: a, b. Engineered: yes. Mutation: yes

Source:

Burkholderia thailandensis. Organism_taxid: 57975. Gene: a8h35_31635. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008

Resolution:

1.40Å R-factor: 0.150 R-free: 0.175

Authors:

T.-P.Cao,J.M.Choi,S.H.Lee

Key ref:

T.-P.Cao et al. Crystal structure of class a b-Lactamase, Penl, Varia asn136asp, From burkholderia thailandensis, In comple ceftazidime-Like boronic acid. To be published, .

Date:

08-Aug-18 Release date: 15-Jan-20

Protein chains

Q2T5A3  (Q2T5A3_BURTA) -  Beta-lactamase from Burkholderia thailandensis (strain ATCC 700388 / DSM 13276 / CCUG 48851 / CIP 106301 / E264)

Seq: 322 a.a.

Struc: 268 a.a.*

* PDB and UniProt seqs differ at 5 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.5.2.6 - beta-lactamase.
• Pathway: Penicillin Biosynthesis and Metabolism
• Reaction: a beta-lactam + H2O = a substituted beta-amino acid
• Cofactor: Zn(2+)