PDBsum entry 6a3b

Transport protein

PDB id: 6a3b

Contents

Protein chains

210 a.a.

129 a.a.

996 a.a.

18 a.a.

Ligands

GTP

EDO

GOL ×2

Metals

_NA

_MG

Waters ×117

PDB id:

6a3b

Name:

Transport protein

Title:

Mvm nes mutant nm13 in complex with crm1-ran-ranbp1

Structure:

Gtp-binding nuclear protein ran. Chain: a. Synonym: androgen receptor-associated protein 24,gtpase ran,ras-like protein tc4,ras-related nuclear protein. Engineered: yes. Mutation: yes. Ran-specific gtpase-activating protein 1. Chain: b. Fragment: lacking c-terminal inhibitory tail and h9 loop.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: ran, ara24, ok/sw-cl.81. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 559292.

Resolution:

2.51Å R-factor: 0.215 R-free: 0.254

Authors:

Q.Sun,Y.Li

Key ref:

M.Sui et al. (2021). Cancer Therapy with Nanoparticle-Medicated Intracellular Expression of Peptide CRM1-Inhibitor. Int J Nanomedicine, 16, 2833-2847. PubMed id: 33883894 DOI: 10.2147/IJN.S266398

Date:

15-Jun-18 Release date: 19-Jun-19

Protein chain

P62826  (RAN_HUMAN) -  GTP-binding nuclear protein Ran from Homo sapiens

Seq: 216 a.a.

Struc: 210 a.a.*

Protein chain

P41920  (YRB1_YEAST) -  Ran-specific GTPase-activating protein 1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 201 a.a.

Struc: 129 a.a.

Protein chain

P30822  (XPO1_YEAST) -  Exportin-1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 1084 a.a.

Struc: 996 a.a.*

Protein chain

P0DJZ2  (NS2_MUMIP) -  Non-structural protein NS2 from Murine minute virus (strain MVM prototype)

Seq: 188 a.a.

Struc: 18 a.a.*

* PDB and UniProt seqs differ at 8 residue positions (black crosses)

Enzyme reactions

• Enzyme class 2: Chain A: E.C.3.6.5.- - ?????
• Enzyme class 3: Chains B, C, D: E.C.?

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

DOI no: 10.2147/IJN.S266398

Int J Nanomedicine 16:2833-2847 (2021)

PubMed id: 33883894

Cancer Therapy with Nanoparticle-Medicated Intracellular Expression of Peptide CRM1-Inhibitor.

M.Sui, M.Xiong, Y.Li, Q.Zhou, X.Shen, D.Jia, M.Gou, Q.Sun.

ABSTRACT

No abstract given.