PDBsum entry 6mdp

Hydrolase

PDB id: 6mdp

Contents

Protein chains

511 a.a.

243 a.a.

17 a.a.

Ligands

ADP ×2

ATP ×9

PDB id:

6mdp

Name:

Hydrolase

Title:

The d1 and d2 domain rings of nsf engaging the snap-25 n-terminus within the 20s supercomplex (focused refinement on d1/d2 rings, class 2)

Structure:

Vesicle-fusing atpase. Chain: a, b, c, d, e, f. Synonym: n-ethylmaleimide-sensitive fusion protein,nem-sensitive fusion protein,vesicular-fusion protein nsf. Engineered: yes. Synaptosomal-associated protein 25. Chain: h. Synonym: snap-25,super protein,sup,synaptosomal-associated 25 kda protein.

Source:

Cricetulus griseus. Chinese hamster. Organism_taxid: 10029. Gene: nsf. Expressed in: escherichia coli. Expression_system_taxid: 562. Rattus norvegicus. Rat. Organism_taxid: 10116.

Authors:

K.I.White,M.Zhao,A.T.Brunger

Key ref:

K.I.White et al. (2018). Structural principles of snare complex recognition by aaa+ protein nsf.. Elife, 7, . PubMed id: 30198481

Date:

04-Sep-18 Release date: 19-Sep-18

Protein chains

P18708  (NSF_CRIGR) -  Vesicle-fusing ATPase from Cricetulus griseus

Seq: 744 a.a.

Struc: 511 a.a.*

Protein chain

P18708  (NSF_CRIGR) -  Vesicle-fusing ATPase from Cricetulus griseus

Seq: 744 a.a.

Struc: 243 a.a.

Protein chain

P60881  (SNP25_RAT) -  Synaptosomal-associated protein 25 from Rattus norvegicus

Seq: 206 a.a.

Struc: 17 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class 2: Chains A, B, C, D, E, F: E.C.3.6.4.6 - vesicle-fusing ATPase.
• Reaction: ATP + H2O = ADP + phosphate + H⁺

ATP (Bound ligand (Het Group name = ATP) corresponds exactly) + H2O = ADP (Bound ligand (Het Group name = ADP) corresponds exactly) + phosphate + H(+)

• Enzyme class 3: Chain H: E.C.?

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site