PDBsum entry 6aso

Splicing

PDB id: 6aso

Contents

Protein chains

369 a.a.

95 a.a.

79 a.a.

59 a.a.

79 a.a.

75 a.a.

67 a.a.

83 a.a.

DNA/RNA

Metals

__K ×2

_MG ×2

_MN

Waters ×134

PDB id:

6aso

Name:

Splicing

Title:

Structure of yeast u6 snrnp with 3'-phosphate terminated u6 RNA

Structure:

U4/u6 snrna-associated-splicing factor prp24. Chain: a. Synonym: u4/u6 snrnp protein. Engineered: yes. U6 snrna-associated sm-like protein lsm2. Chain: b. Synonym: small nuclear ribonucleoprotein d homolog snp3. Engineered: yes. U6 snrna-associated sm-like protein lsm3.

Source:

Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 4932. Strain: atcc 204508 / s288c. Gene: prp24, ymr268c, ym8156.10c. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: lsm2, smx5, snp3, ybl026w, ybl0425. Gene: lsm3, smx4, uss2, ylr438c-a.

Resolution:

2.71Å R-factor: 0.199 R-free: 0.247

Authors:

E.J.Montemayor,D.A.Brow,S.E.Butcher

Key ref:

E.J.Montemayor et al. (2018). Architecture of the U6 snRNP reveals specific recognition of 3'-end processed U6 snRNA. Nat Commun, 9, 1749. PubMed id: 29717126

Date:

25-Aug-17 Release date: 09-May-18

Protein chain

P49960  (PRP24_YEAST) -  U4/U6 snRNA-associated-splicing factor PRP24 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 444 a.a.

Struc: 369 a.a.

Protein chain

P38203  (LSM2_YEAST) -  LSM complex subunit LSM2 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 95 a.a.

Struc: 95 a.a.

Protein chain

P57743  (LSM3_YEAST) -  LSM complex subunit LSM3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 89 a.a.

Struc: 79 a.a.

Protein chain

P40070  (LSM4_YEAST) -  LSM complex subunit LSM4 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 187 a.a.

Struc: 59 a.a.

Protein chain

P40089  (LSM5_YEAST) -  LSM complex subunit LSM5 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 93 a.a.

Struc: 79 a.a.

Protein chain

A6ZYX7  (LSM6_YEAS7) -  U6 snRNA-associated Sm-like protein LSm6 from Saccharomyces cerevisiae (strain YJM789)

Seq: 86 a.a.

Struc: 75 a.a.

Protein chain

P53905  (LSM7_YEAST) -  LSM complex subunit LSM7 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 115 a.a.

Struc: 67 a.a.

Protein chain

P47093  (LSM8_YEAST) -  LSM2-LSM8 complex subunit LSM8 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 109 a.a.

Struc: 83 a.a.

DNA/RNA chain

C-A-A-U-U-U-G-A-A-A-C-A-A-U-A-C-A-G-A-G-A-U-G-A-U-C-A-G-C-G-G-U-U-C-C-C-C-U-G- 70 bases

Nat Commun 9:1749 (2018)

PubMed id: 29717126

Architecture of the U6 snRNP reveals specific recognition of 3'-end processed U6 snRNA.

E.J.Montemayor, A.L.Didychuk, A.D.Yake, G.K.Sidhu, D.A.Brow, S.E.Butcher.

ABSTRACT

The spliceosome removes introns from precursor messenger RNA (pre-mRNA) to produce mature mRNA. Prior to catalysis, spliceosomes are assembled de novo onto pre-mRNA substrates. During this assembly process, U6 small nuclear RNA (snRNA) undergoes extensive structural remodeling. The early stages of this remodeling process are chaperoned by U6 snRNP proteins Prp24 and the Lsm2-8 heteroheptameric ring. We now report a structure of the U6 snRNP from Saccharomyces cerevisiae. The structure reveals protein-protein contacts that position Lsm2-8 in close proximity to the chaperone "active site" of Prp24. The structure also shows how the Lsm2-8 ring specifically recognizes U6 snRNA that has been post-transcriptionally modified at its 3' end, thereby elucidating the mechanism by which U6 snRNPs selectively recruit 3' end-processed U6 snRNA into spliceosomes. Additionally, the structure reveals unanticipated homology between the C-terminal regions of Lsm8 and the cytoplasmic Lsm1 protein involved in mRNA decay.