PDBsum entry 5w3c

Unknown function

PDB id: 5w3c

Contents

Protein chain

171 a.a.

Ligands

LFA

SO4 ×2

SEC

Waters ×19

PDB id:

5w3c

Name:

Unknown function

Title:

Crystal structure of mutant cj ycei protein (cj-n182c) with selenocysteine guest structure

Structure:

Polyisoprenoid-binding protein. Chain: a. Fragment: unp residues 22-190. Synonym: ycei periplasmic protein, protein ycei, periplasmic protein. Engineered: yes. Mutation: yes

Source:

Campylobacter jejuni. Organism_taxid: 197. Gene: a0l11_08945, a0m64_02260, a0m70_03345, ad53_02580. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

2.87Å R-factor: 0.235 R-free: 0.274

Authors:

T.R.Huber,C.D.Snow

Key ref:

T.R.Huber et al. (2018). Installing Guest Molecules at Specific Sites within Scaffold Protein Crystals. Bioconjug Chem, 29, 17-22. PubMed id: 29232505 DOI: 10.1021/acs.bioconjchem.7b00668

Date:

07-Jun-17 Release date: 03-Jan-18

Protein chain

Q0PB90  (Q0PB90_CAMJE) -  Periplasmic protein from Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)

Seq: 190 a.a.

Struc: 171 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

DOI no: 10.1021/acs.bioconjchem.7b00668

Bioconjug Chem 29:17-22 (2018)

PubMed id: 29232505

Installing Guest Molecules at Specific Sites within Scaffold Protein Crystals.

T.R.Huber, E.C.McPherson, C.E.Keating, C.D.Snow.

ABSTRACT

Protein crystals are porous self-assembling materials that can be rapidly evolved by mutagenesis. We aimed to develop scaffold assisted crystallography techniques in an engineered protein crystal with large pores (>13 nm). Guest molecules were installed via a single covalent bond to attempt to reduce the conformational freedom and achieve high-occupancy structures. We used four different conjugation strategies to attach guest molecules to three different cysteine sites within pre-existing protein crystals. In all but one case, the presence of the adduct was obvious in the electron density. Structure determination of larger guest molecules may be feasible due to the large pores of the engineered scaffold crystals.