PDBsum entry 5vsr

Transferase

PDB id: 5vsr

Contents

Protein chains

175 a.a.

306 a.a.

Ligands

AF0

Metals

_MG ×3

Waters ×18

PDB id:

5vsr

Name:

Transferase

Title:

Aba-mimicking ligand amf4 in complex with aba receptor pyl2 and pp2c hab1

Structure:

Abscisic acid receptor pyl2. Chain: a. Synonym: pyr1-like protein 2,regulatory components of aba receptor 14. Engineered: yes. Protein phosphatase 2c 16. Chain: b. Synonym: atpp2c16,atp2c-ha,protein hypersensitive to aba 1,protein phosphatase 2c hab1,pp2c hab1.

Source:

Arabidopsis thaliana. Mouse-ear cress. Organism_taxid: 3702. Gene: pyl2, rcar14, at2g26040, t19l18.15. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: hab1, p2c-ha, at1g72770, f28p22.4. Expression_system_taxid: 562

Resolution:

2.62Å R-factor: 0.242 R-free: 0.275

Authors:

M.-J.Cao,Y.-L.Zhang,X.Liu,H.Huang,X.E.Zhou,W.-L.Wang,A.Zeng,C.- Z.Zhao,T.Si,J.-M.Du,W.-W.Wu,F.-X.Wang,H.X.Xu,J.-K.Zhu

Key ref:

M.J.Cao et al. (2017). Combining chemical and genetic approaches to increase drought resistance in plants. Nat Commun, 8, 1183. PubMed id: 29084945

Date:

12-May-17 Release date: 15-Nov-17

Protein chain

O80992  (PYL2_ARATH) -  Abscisic acid receptor PYL2 from Arabidopsis thaliana

Seq: 190 a.a.

Struc: 175 a.a.*

Protein chain

Q9CAJ0  (P2C16_ARATH) -  Protein phosphatase 2C 16 from Arabidopsis thaliana

Seq: 511 a.a.

Struc: 306 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class 1: Chain A: E.C.?
• Enzyme class 2: Chain B: E.C.3.1.3.16 - protein-serine/threonine phosphatase.
• Reaction:
  1. O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate
  2. O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

Nat Commun 8:1183 (2017)

PubMed id: 29084945

Combining chemical and genetic approaches to increase drought resistance in plants.

M.J.Cao, Y.L.Zhang, X.Liu, H.Huang, X.E.Zhou, W.L.Wang, A.Zeng, C.Z.Zhao, T.Si, J.Du, W.W.Wu, F.X.Wang, H.E.Xu, J.K.Zhu.

ABSTRACT

Drought stress is a major threat to crop production, but effective methods to mitigate the adverse effects of drought are not available. Here, we report that adding fluorine atoms in the benzyl ring of the abscisic acid (ABA) receptor agonist AM1 optimizes its binding to ABA receptors by increasing the number of hydrogen bonds between the compound and the surrounding amino acid residues in the receptor ligand-binding pocket. The new chemicals, known as AMFs, have long-lasting effects in promoting stomatal closure and inducing the expression of stress-responsive genes. Application of AMFs or transgenic overexpression of the receptor PYL2 in Arabidopsis and soybean plants confers increased drought resistance. The greatest increase in drought resistance is achieved when AMFs are applied to the PYL2-overexpression transgenic plants. Our results demonstrate that the combining of potent chemicals with transgenic overexpression of an ABA receptor is very effective in helping plants combat drought stress.