PDBsum entry 5uwp

Protein transport

PDB id: 5uwp

Contents

Protein chains

209 a.a.

128 a.a.

996 a.a.

11 a.a.

Ligands

GNP

GOL ×3

Metals

_MG

Waters ×889

PDB id:

5uwp

Name:

Protein transport

Title:

Crystal structure of mdia2 nes peptide in complex with crm1-ran-ranbp1

Structure:

Gtp-binding nuclear protein ran. Chain: a. Synonym: androgen receptor-associated protein 24, gtpase ran, ras- like protein tc4, ras-related nuclear protein. Engineered: yes. Ran-specific gtpase-activating protein 1. Chain: b. Synonym: chromosome stability protein 20, perinuclear array-localized protein, ran-binding protein 1, ranbp1.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: ran, ara24, ok/sw-cl.81. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 4932.

Resolution:

2.05Å R-factor: 0.179 R-free: 0.213

Authors:

H.Y.J.Fung,Y.M.Chook

Key ref:

H.Y.Fung et al. (2017). Nuclear export receptor crm1 recognizes diverse conformations in nuclear export signals.. Elife, 6, . PubMed id: 28282025

Date:

21-Feb-17 Release date: 22-Mar-17

Protein chain

P62826  (RAN_HUMAN) -  GTP-binding nuclear protein Ran from Homo sapiens

Seq: 216 a.a.

Struc: 209 a.a.

Protein chain

P41920  (YRB1_YEAST) -  Ran-specific GTPase-activating protein 1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 201 a.a.

Struc: 128 a.a.

Protein chain

P30822  (XPO1_YEAST) -  Exportin-1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 1084 a.a.

Struc: 996 a.a.*

Protein chain

Q9NSV4  (DIAP3_HUMAN) -  Protein diaphanous homolog 3 from Homo sapiens

Seq: 1193 a.a.

Struc: 11 a.a.

* PDB and UniProt seqs differ at 5 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chain A: E.C.3.6.5.- - ?????