PDBsum entry 5ugv

Oxidoreductase

PDB id: 5ugv

Contents

Protein chains

245 a.a.

Ligands

NAD ×2

SO4

PGE ×2

Waters ×91

PDB id:

5ugv

Name:

Oxidoreductase

Title:

Dapb from mycobacterium tuberculosis

Structure:

4-hydroxy-tetrahydrodipicolinate reductase. Chain: a, b. Synonym: htpa reductase. Engineered: yes

Source:

Mycobacterium tuberculosis (strain atcc 25177 / h37ra). Organism_taxid: 419947. Strain: atcc 25177 / h37ra. Gene: dapb, mra_2798. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

2.25Å R-factor: 0.167 R-free: 0.200

Authors:

S.P.Pote,N.Mank,M.Chruszcz

Key ref:

S.P.Pote et al. Dapb from mycobacterium tuberculosis. To be published, . PubMed id: 32980502

Date:

10-Jan-17 Release date: 17-Jan-18

Protein chains

P9WP23  (DAPB_MYCTU) -  4-hydroxy-tetrahydrodipicolinate reductase from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

Seq: 245 a.a.

Struc: 245 a.a.

Enzyme reactions

• Enzyme class: E.C.1.17.1.8 - 4-hydroxy-tetrahydrodipicolinate reductase.
• Reaction:
  1. (S)-2,3,4,5-tetrahydrodipicolinate + NAD⁺ + H2O = (2S,4S)-4- hydroxy-2,3,4,5-tetrahydrodipicolinate + NADH + H⁺
  2. (S)-2,3,4,5-tetrahydrodipicolinate + NADP⁺ + H2O = (2S,4S)-4- hydroxy-2,3,4,5-tetrahydrodipicolinate + NADPH + H⁺

(S)-2,3,4,5-tetrahydrodipicolinate + NAD(+) + H2O (Bound ligand (Het Group name = NAD) matches with 79.55% similarity) = (2S,4S)-4- hydroxy-2,3,4,5-tetrahydrodipicolinate + NADH + H(+)

(S)-2,3,4,5-tetrahydrodipicolinate + NADP(+) + H2O (Bound ligand (Het Group name = NAD) matches with 72.92% similarity) = (2S,4S)-4- hydroxy-2,3,4,5-tetrahydrodipicolinate + NADPH + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site