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PDBsum entry 5tss
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Contents |
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* Residue conservation analysis
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PDB id:
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Toxin
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Title:
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Toxic shock syndrome toxin-1: orthorhombic p222(1) crystal form
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Structure:
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Toxic shock syndrome toxin-1. Chain: a, b. Synonym: tsst-1
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Source:
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Staphylococcus aureus. Organism_taxid: 1280. Strain: mn 8. Cellular_location: secreted
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Resolution:
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Authors:
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G.S.Prasad,R.Radhakrishnan,D.T.Mitchell,C.A.Earhart,M.M.Dinges, W.J.Cook,P.M.Schlivert,D.H.Ohlendorf
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Key ref:
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G.S.Prasad
et al.
(1997).
Refined structures of three crystal forms of toxic shock syndrome toxin-1 and of a tetramutant with reduced activity.
Protein Sci,
6,
1220-1227.
PubMed id:
DOI:
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Date:
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11-Dec-96
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Release date:
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24-Dec-97
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PROCHECK
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Headers
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References
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P06886
(TSST_STAAU) -
Toxic shock syndrome toxin-1 from Staphylococcus aureus
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Seq:
Struc:
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234 a.a.
194 a.a.*
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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*
PDB and UniProt seqs differ
at 1 residue position (black
cross)
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DOI no:
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Protein Sci
6:1220-1227
(1997)
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PubMed id:
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Refined structures of three crystal forms of toxic shock syndrome toxin-1 and of a tetramutant with reduced activity.
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G.S.Prasad,
R.Radhakrishnan,
D.T.Mitchell,
C.A.Earhart,
M.M.Dinges,
W.J.Cook,
P.M.Schlievert,
D.H.Ohlendorf.
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ABSTRACT
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The structure of toxic shock syndrome toxin-1 (TSST-1), the causative agent in
toxic shock syndrome, has been determined in three crystal forms. The three
structural models have been refined to R-factors of 0.154, 0.150, and 0.198 at
resolutions of 2.05 A, 2.90 A, and 2.75 A, respectively. One crystal form of
TSST-1 contains a zinc ion bound between two symmetry-related molecules.
Although not required for biological activity, zinc dramatically potentiates the
mitogenicity of TSST-1 at very low concentrations. In addition, the structure of
the tetramutant TSST-1H [T69I, Y80W, E132K, I140T], which is nonmitogenic and
does not amplify endotoxin shock, has been determined and refined in a fourth
crystal form (R-factor = 0.173 to 1.9 A resolution).
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Selected figure(s)
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Figure 1.
Fig. 1. Front view of TSST-I Numbers refer to /3 strands: letters refer to
helices. Key structural features are shown extracted from the rest of the
molecule.
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Figure 2.
Fig. 2. 2F, - F, electrondensitymapscontouredat I n. (A) RegionaroundPhe 131 in C2221 crystal form. Thesidechainbeyond
Cg was mittedfromthepositionalrefinementandphasecalculation. (B) Regionaround Zn 206 in P4,2)2 crystalform.Symmetry-
relatedmolecule is gray.
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The above figures are
reprinted
from an Open Access publication published by the Protein Society:
Protein Sci
(1997,
6,
1220-1227)
copyright 1997.
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Figures were
selected
by an automated process.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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S.J.Li,
D.L.Hu,
E.K.Maina,
K.Shinagawa,
K.Omoe,
and
A.Nakane
(2011).
Superantigenic activity of toxic shock syndrome toxin-1 is resistant to heating and digestive enzymes.
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J Appl Microbiol,
110,
729-736.
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A.Zemla,
B.Geisbrecht,
J.Smith,
M.Lam,
B.Kirkpatrick,
M.Wagner,
T.Slezak,
and
C.E.Zhou
(2007).
STRALCP--structure alignment-based clustering of proteins.
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Nucleic Acids Res,
35,
e150.
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B.Moza,
A.K.Varma,
R.A.Buonpane,
P.Zhu,
C.A.Herfst,
M.J.Nicholson,
A.K.Wilbuer,
N.P.Seth,
K.W.Wucherpfennig,
J.K.McCormick,
D.M.Kranz,
and
E.J.Sundberg
(2007).
Structural basis of T-cell specificity and activation by the bacterial superantigen TSST-1.
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EMBO J,
26,
1187-1197.
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PDB code:
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J.W.Shupp,
M.Jett,
and
C.H.Pontzer
(2002).
Identification of a transcytosis epitope on staphylococcal enterotoxins.
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Infect Immun,
70,
2178-2186.
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M.M.Dinges,
P.M.Orwin,
and
P.M.Schlievert
(2000).
Exotoxins of Staphylococcus aureus.
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Clin Microbiol Rev,
13,
16.
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W.W.Kum,
K.B.Laupland,
and
A.W.Chow
(2000).
Defining a novel domain of staphylococcal toxic shock syndrome toxin-1 critical for major histocompatibility complex class II binding, superantigenic activity, and lethality.
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Can J Microbiol,
46,
171-179.
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C.A.Earhart,
D.T.Mitchell,
D.L.Murray,
D.M.Pinheiro,
M.Matsumura,
P.M.Schlievert,
and
D.H.Ohlendorf
(1998).
Structures of five mutants of toxic shock syndrome toxin-1 with reduced biological activity.
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Biochemistry,
37,
7194-7202.
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PDB codes:
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M.Kotb
(1998).
Superantigens of gram-positive bacteria: structure-function analyses and their implications for biological activity.
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Curr Opin Microbiol,
1,
56-65.
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
code is
shown on the right.
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Links
