PDBsum entry 5trc

Ligase

PDB id

5trc

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Contents

			Protein chains

					426 a.a.


					398 a.a.

			Metals

					_CL ×2

PDB id:

5trc

Links

Name:

Ligase

Title:

Crystal structure of phosphorylated ac3-ac5 domains of yeast acetyl- coa carboxylase

Structure:

Acetyl-coa carboxylase. Chain: a, b. Synonym: acc,fatty acid synthetase 3,mRNA transport-defective protein 7. Engineered: yes

Source:

Saccharomyces cerevisiae (strain atcc 204508 / s288c). Baker's yeast. Organism_taxid: 559292. Strain: atcc 204508 / s288c. Gene: acc1, abp2, fas3, mtr7, ynr016c, n3175. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

2.90Å

R-factor:

0.226

R-free:

0.287

Authors:

J.Wei,L.Tong

Key ref:

J.Wei et al. (2016). A unified molecular mechanism for the regulation of acetyl-CoA carboxylase by phosphorylation. Cell Discov, 2, 16044. PubMed id: 27990296 DOI: 10.1038/celldisc.2016.44

Date:

26-Oct-16

Release date:

07-Dec-16

PROCHECK

	Headers

	References

Protein chain

Q00955 (ACAC_YEAST) - Acetyl-CoA carboxylase from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: Struc:

Seq: Struc:

Seq: Struc:

Seq: Struc:

Seq: Struc:					2233 a.a. 426 a.a.

Protein chain

Q00955 (ACAC_YEAST) - Acetyl-CoA carboxylase from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: Struc:

Seq: Struc:

Seq: Struc:

Seq: Struc:

Seq: Struc:					2233 a.a. 398 a.a.

Key:

PfamA domain

Secondary structure



		Enzyme reactions

Enzyme class 2:

Chains A, B: E.C.6.3.4.14 - biotin carboxylase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

N₆-biotinyl-L-lysyl-[protein] + hydrogencarbonate + ATP = N₆- carboxybiotinyl-L-lysyl-[protein] + ADP + phosphate + H⁺

N(6)-biotinyl-L-lysyl-[protein]	+	hydrogencarbonate	+	ATP	=	N(6)- carboxybiotinyl-L-lysyl-[protein]	+	ADP	+	phosphate	+	H(+)

Enzyme class 3:

Chains A, B: E.C.6.4.1.2 - acetyl-CoA carboxylase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

hydrogencarbonate + acetyl-CoA + ATP = malonyl-CoA + ADP + phosphate + H⁺

hydrogencarbonate	+	acetyl-CoA	+	ATP	=	malonyl-CoA	+	ADP	+	phosphate	+	H(+)

Cofactor:

Biotin

Biotin

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

DOI no: 10.1038/celldisc.2016.44	Cell Discov 2:16044 (2016)
PubMed id: 27990296

A unified molecular mechanism for the regulation of acetyl-CoA carboxylase by phosphorylation.
J.Wei, Y.Zhang, T.Y.Yu, K.Sadre-Bazzaz, M.J.Rudolph, G.A.Amodeo, L.S.Symington, T.Walz, L.Tong.

ABSTRACT

No abstract given.