PDBsum entry 5tdh

Cell cycle

PDB id: 5tdh

Contents

Protein chains

337 a.a.

339 a.a.

55 a.a.

Ligands

GDP ×2

PDB id:

5tdh

Name:

Cell cycle

Title:

The crystal structure of the dominant negative mutant g protein alpha(i)-1-beta-1-gamma-2 g203a/a326s

Structure:

Guanine nucleotide-binding protein g(i) subunit alpha-1. Chain: a, h. Synonym: adenylate cyclase-inhibiting g alpha protein. Engineered: yes. Mutation: yes. Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1. Chain: b, j. Synonym: g-beta-1,transducin beta chain 1.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: gnai1. Expressed in: escherichia coli. Expression_system_taxid: 562. Rattus norvegicus. Rat. Organism_taxid: 10116.

Resolution:

3.00Å R-factor: 0.278 R-free: 0.305

Authors:

P.Liu,M.-Z.Jia,X.E.Zhou,P.W.De Waal,B.M.Dickson,B.Liu,L.Hou,Y.-T.Yin, Y.-Y.Kang,Y.Shi,K.Melcher,H.E.Xu,Y.Jiang

Key ref:

P.Liu et al. (2016). The structural basis of the dominant negative phenotype of the Gαi1β1γ2 G203A/A326S heterotrimer. Acta Pharmacol Sin, 37, 1259-1272. PubMed id: 27498775

Date:

19-Sep-16 Release date: 09-Nov-16

Protein chains

P63096  (GNAI1_HUMAN) -  Guanine nucleotide-binding protein G(i) subunit alpha-1 from Homo sapiens

Seq: 354 a.a.

Struc: 337 a.a.*

Protein chains

P54311  (GBB1_RAT) -  Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 from Rattus norvegicus

Seq: 340 a.a.

Struc: 339 a.a.

Protein chains

P63212  (GBG2_BOVIN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 from Bos taurus

Seq: 71 a.a.

Struc: 55 a.a.

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chains A, B, G, H, J, K: E.C.?

Acta Pharmacol Sin 37:1259-1272 (2016)

PubMed id: 27498775

The structural basis of the dominant negative phenotype of the Gαi1β1γ2 G203A/A326S heterotrimer.

P.Liu, M.Z.Jia, X.E.Zhou, P.W.De Waal, B.M.Dickson, B.Liu, L.Hou, Y.T.Yin, Y.Y.Kang, Y.Shi, K.Melcher, H.E.Xu, Y.Jiang.

ABSTRACT

No abstract given.