PDBsum entry 5tcs

Replication

PDB id: 5tcs

Contents

Protein chains

266 a.a.

211 a.a.

97 a.a.

114 a.a.

Metals

_MG

Waters ×39

PDB id:

5tcs

Name:

Replication

Title:

Crystal structure of a dwarf ndc80 tetramer

Structure:

Kinetochore protein ndc80. Chain: a. Synonym: 80 kda spindle component protein,nuclear division cycle protein 80,two-hybrid interaction with dmc1 protein 3,80 kda spindle component protein,nuclear division cycle protein 80,two-hybrid interaction with dmc1 protein 3. Engineered: yes. Kinetochore protein nuf2. Chain: b.

Source:

Saccharomyces cerevisiae (strain atcc 204508 / s288c). Baker's yeast. Organism_taxid: 559292. Strain: atcc 204508 / s288c. Gene: ndc80, hec1, tid3, yil144w. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: nuf2, yol069w.

Resolution:

2.83Å R-factor: 0.240 R-free: 0.266

Authors:

R.Valverde,S.C.Harrison

Key ref:

R.Valverde et al. (2016). Conserved Tetramer Junction in the Kinetochore Ndc80 Complex. Cell Rep, 17, 1915-1922. PubMed id: 27851957 DOI: 10.1016/j.celrep.2016.10.065

Date:

15-Sep-16 Release date: 23-Nov-16

Protein chain

P40460  (NDC80_YEAST) -  Kinetochore protein NDC80 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 691 a.a.

Struc: 266 a.a.*

Protein chain

P33895  (NUF2_YEAST) -  Kinetochore protein NUF2 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 451 a.a.

Struc: 211 a.a.*

Protein chain

Q04477  (SPC24_YEAST) -  Kinetochore protein SPC24 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 213 a.a.

Struc: 97 a.a.

Protein chain

P40014  (SPC25_YEAST) -  Kinetochore protein SPC25 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 221 a.a.

Struc: 114 a.a.*

* PDB and UniProt seqs differ at 120 residue positions (black crosses)

DOI no: 10.1016/j.celrep.2016.10.065

Cell Rep 17:1915-1922 (2016)

PubMed id: 27851957

Conserved Tetramer Junction in the Kinetochore Ndc80 Complex.

R.Valverde, J.Ingram, S.C.Harrison.

ABSTRACT

No abstract given.