O.Aurelius
et al.
(2017).
Long-wavelength macromolecular crystallography - First successful native SAD experiment close to the sulfur edge.
Nucl Instrum Methods Phys Res B,
411,
12-16.
PubMed id: 29276323
Phasing of novel macromolecular crystal structures has been challenging since
the start of structural biology. Making use of anomalous diffraction of natively
present elements, such as sulfur and phosphorus, for phasing has been possible
for some systems, but hindered by the necessity to access longer X-ray
wavelengths in order to make most use of the anomalous scattering contributions
of these elements. Presented here are the results from a first successful
experimental phasing study of a macromolecular crystal structure at a wavelength
close to the sulfur K edge. This has been made possible by the in-vacuum setup
and the long-wavelength optimised experimental setup at the I23 beamline at
Diamond Light Source. In these early commissioning experiments only standard
data collection and processing procedures have been applied, in particular no
dedicated absorption correction has been used. Nevertheless the success of the
experiment demonstrates that the capability to extract phase information can be
even further improved once data collection protocols and data processing have
been optimised.
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