PDBsum entry 5t3a

Hydrolase

PDB id: 5t3a

Contents

Protein chain

212 a.a.

Ligands

ACT ×3

MES

Waters ×46

PDB id:

5t3a

Name:

Hydrolase

Title:

Maedi-visna virus (mvv) integrase ccd-ctd (residues 60-275)

Structure:

Integrase. Chain: a. Engineered: yes

Source:

Visna/maedi virus. Mvv. Organism_taxid: 11741. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

2.50Å R-factor: 0.182 R-free: 0.240

Authors:

N.J.Cook,V.E.Pye,P.Cherepanov

Key ref:

A.Ballandras-Colas et al. (2017). A supramolecular assembly mediates lentiviral DNA integration. Science, 355, 93-95. PubMed id: 28059770 DOI: 10.1126/science.aah7002

Date:

25-Aug-16 Release date: 18-Jan-17

Protein chain

P35956  (POL_VILVK) -  Gag-Pol polyprotein from Maedi visna virus (strain KV1772)

Seq: 1506 a.a.

Struc: 212 a.a.

Enzyme reactions

• Enzyme class 1: E.C.2.7.7.- - ?????
• Enzyme class 2: E.C.2.7.7.49 - RNA-directed Dna polymerase.
• Reaction: DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate
• Enzyme class 3: E.C.2.7.7.7 - DNA-directed Dna polymerase.
• Reaction: DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate
• Enzyme class 4: E.C.3.1.-.-
• Enzyme class 5: E.C.3.1.13.2 - exoribonuclease H.
• Reaction: Exonucleolytic cleavage to 5'-phosphomonoester oligonucleotides in both 5'- to 3'- and 3'- to 5'-directions.
• Enzyme class 6: E.C.3.1.26.4 - ribonuclease H.
• Reaction: Endonucleolytic cleavage to 5'-phosphomonoester.
• Enzyme class 7: E.C.3.4.23.- - ?????
• Enzyme class 8: E.C.3.6.1.23 - dUTP diphosphatase.
• Reaction: dUTP + H2O = dUMP + diphosphate + H⁺

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

DOI no: 10.1126/science.aah7002

Science 355:93-95 (2017)

PubMed id: 28059770

A supramolecular assembly mediates lentiviral DNA integration.

A.Ballandras-Colas, D.P.Maskell, E.Serrao, J.Locke, P.Swuec, S.R.Jónsson, A.Kotecha, N.J.Cook, V.E.Pye, I.A.Taylor, V.Andrésdóttir, A.N.Engelman, A.Costa, P.Cherepanov.

ABSTRACT

No abstract given.