PDBsum entry 5ssx

Oxidoreductase

PDB id: 5ssx

Contents

Protein chain

278 a.a.

Ligands

NAG-NAG

_CA ×2

Metals

CU1

Waters ×430

PDB id:

5ssx

Name:

Oxidoreductase

Title:

Crystal structure human formylglycine generating enzyme e130d mutant

Structure:

Formylglycine-generating enzyme. Chain: a. Synonym: fgE,C-alpha-formylglycine-generating enzyme 1,sulfatase- modifying factor 1. Engineered: yes. Mutation: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: sumf1, psec0152, unq3037/pro9852. Expressed in: trichoplusia ni. Expression_system_taxid: 7111. Expression_system_cell_line: hi5.

Resolution:

1.02Å R-factor: 0.124 R-free: 0.138

Authors:

K.Radhakrishnan,L.Schlotawa,M.G.Rudolph

Key ref:

J.Kowal et al. Crystal structure of human formylglycine generating e e130d mutant. To be published, .

Date:

12-Aug-22 Release date: 23-Aug-23

Protein chain

Q8NBK3  (SUMF1_HUMAN) -  Formylglycine-generating enzyme from Homo sapiens

Seq: 374 a.a.

Struc: 278 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.1.8.3.7 - formylglycine-generating enzyme.
• Reaction: L-cysteinyl-[sulfatase] + 2 a thiol + O2 = an organic disulfide + 3-oxo- L-alanyl-[sulfatase] + hydrogen sulfide + H2O + H⁺
• Cofactor: Ca(2+); Cu cation

Molecule diagrams generated from .mol files obtained from the KEGG ftp site