PDBsum entry 5q0c

Hydrolase/hydrolase inhibitor

PDB id: 5q0c

Contents

Protein chains

(+ 2 more) 328 a.a.

Ligands

96G ×8

FDP ×8

Waters ×545

PDB id:

5q0c

Name:

Hydrolase/hydrolase inhibitor

Title:

Human liver fructose-1,6-bisphosphatase 1 (fructose 1,6-bisphosphate 1-phosphatase, E.C.3.1.3.11) complexed with the allosteric inhibitor 1-(5-bromo-1,3-thiazol-2-yl)-3-[5-(4-methoxyphenyl)thiophen-2- yl]sulfonylurea and with fructose-2,6-diphosphate

Structure:

Fructose-1,6-bisphosphatase isozyme 2. Chain: a, b, c, d, e, f, g, h. Synonym: fbpase 2,d-fructose-1,6-bisphosphate 1-phosphohydrolase 2, muscle fbpase. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: fbp2. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

2.40Å R-factor: 0.170 R-free: 0.210

Authors:

A.Ruf,C.Joseph,A.Alker,D.Banner,T.Tetaz,J.Benz,B.Kuhn,M.G.Rudolph, H.Yang,C.Shao,S.K.Burley

Key ref:

A.Ruf et al. Human liver fructose-1,6-Bisphosphatase 1 (fructose 1,6-Bisphosphate 1-Phosphatase, E.C.3.1.3.11) complex the allosteric inhibitor 1-(5-Bromo-1,3-Thiazol-2-Yl)-3-[5-(4-Methoxyphenyl) thiophen-2-Yl]sulfonylurea and with f2,6p. To be published, .

Date:

18-Apr-17 Release date: 09-Jan-19

Protein chains

O00757  (F16P2_HUMAN) -  Fructose-1,6-bisphosphatase isozyme 2 from Homo sapiens

Seq: 339 a.a.

Struc: 328 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.3.1.3.11 - fructose-bisphosphatase.
• Pathway: Pentose Phosphate Pathway (later stages)
• Reaction: beta-D-fructose 1,6-bisphosphate + H2O = beta-D-fructose 6-phosphate + phosphate

beta-D-fructose 1,6-bisphosphate + H2O = beta-D-fructose 6-phosphate (Bound ligand (Het Group name = FDP) matches with 80.00% similarity) + phosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site