PDBsum entry 5oaf

Gene regulation

PDB id: 5oaf

Contents

Protein chains

436 a.a.

418 a.a.

Ligands

ADP ×6

PDB id:

5oaf

Name:

Gene regulation

Title:

Human rvb1/rvb2 heterohexamer in ino80 complex

Structure:

Ruvb-like 1. Chain: a, c, e. Synonym: 49 kda tata box-binding protein-interacting protein,49 kda tbp-interacting protein,54 kda erythrocyte cytosolic protein,ecp-54, ino80 complex subunit h,nuclear matrix protein 238,nmp 238,pontin 52, tip49a,tip60-associated protein 54-alpha,tap54-alpha. Engineered: yes. Ruvb-like 2. Chain: b, d, f.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: ruvbl1, ino80h, nmp238, tip49, tip49a. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Gene: ruvbl2, ino80j, tip48, tip49b, cgi-46.

Authors:

R.J.Aramayo,R.Bythell-Douglas,R.Ayala,O.Willhoft,D.Wigley,X.Zhang

Key ref:

R.J.Aramayo et al. (2018). Cryo-EM structures of the human INO80 chromatin-remodeling complex. Nat Struct Mol Biol, 25, 37-44. PubMed id: 29323271

Date:

21-Jun-17 Release date: 13-Dec-17

Protein chains

Q9Y265  (RUVB1_HUMAN) -  RuvB-like 1 from Homo sapiens

Seq: 456 a.a.

Struc: 436 a.a.

Protein chains

Q9Y230  (RUVB2_HUMAN) -  RuvB-like 2 from Homo sapiens

Seq: 463 a.a.

Struc: 418 a.a.

Enzyme reactions

• Enzyme class: Chains A, B, C, D, E, F: E.C.3.6.4.12 - Dna helicase.
• Reaction: ATP + H2O = ADP + phosphate + H⁺

ATP + H2O = ADP (Bound ligand (Het Group name = ADP) corresponds exactly) + phosphate + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

Nat Struct Mol Biol 25:37-44 (2018)

PubMed id: 29323271

Cryo-EM structures of the human INO80 chromatin-remodeling complex.

R.J.Aramayo, O.Willhoft, R.Ayala, R.Bythell-Douglas, D.B.Wigley, X.Zhang.

ABSTRACT

Access to chromatin for processes such as transcription and DNA repair requires the sliding of nucleosomes along DNA. This process is aided by chromatin-remodeling complexes, such as the multisubunit INO80 chromatin-remodeling complex. Here we present cryo-EM structures of the active core complex of human INO80 at 9.6 Å, with portions at 4.1-Å resolution, and reconstructions of combinations of subunits. Together, these structures reveal the architecture of the INO80 complex, including Ino80 and actin-related proteins, which is assembled around a single RUVBL1 (Tip49a) and RUVBL2 (Tip49b) AAA+ heterohexamer. An unusual spoked-wheel structural domain of the Ino80 subunit is engulfed by this heterohexamer; both, in combination, form the core of the complex. We also identify a cleft in RUVBL1 and RUVBL2, which forms a major interaction site for partner proteins and probably communicates these interactions to its nucleotide-binding sites.