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PDBsum entry 5o64
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Membrane protein
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PDB id
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5o64
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Contents |
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332 a.a.
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249 a.a.
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273 a.a.
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323 a.a.
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PDB id:
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| Name: |
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Membrane protein
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Title:
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From macrocrystals to microcrystals: a strategy for membrane protein serial crystallography
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Structure:
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Photosynthetic reaction center cytochromE C subunit. Chain: c. Synonym: cytochrome c558/c559. Reaction center protein h chain. Chain: h. Synonym: photosynthetic reaction center h subunit. Reaction center protein l chain. Chain: l. Synonym: photosynthetic reaction center l subunit.
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Source:
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Blastochloris viridis. Organism_taxid: 1079. Organism_taxid: 1079
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Resolution:
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3.30Å
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R-factor:
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0.156
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R-free:
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0.196
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Authors:
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R.Dods,P.Baath,G.Branden,R.Neutze
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Key ref:
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R.Dods
et al.
(2017).
From Macrocrystals to Microcrystals: A Strategy for Membrane Protein Serial Crystallography.
Structure,
25,
1461.
PubMed id:
DOI:
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Date:
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05-Jun-17
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Release date:
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16-Aug-17
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PROCHECK
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Headers
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References
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P07173
(CYCR_BLAVI) -
Photosynthetic reaction center cytochrome c subunit from Blastochloris viridis
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Seq:
Struc:
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356 a.a.
332 a.a.
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P06008
(RCEH_BLAVI) -
Reaction center protein H chain from Blastochloris viridis
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Seq:
Struc:
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258 a.a.
249 a.a.
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DOI no:
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Structure
25:1461
(2017)
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PubMed id:
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From Macrocrystals to Microcrystals: A Strategy for Membrane Protein Serial Crystallography.
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R.Dods,
P.Båth,
D.Arnlund,
K.R.Beyerlein,
G.Nelson,
M.Liang,
R.Harimoorthy,
P.Berntsen,
E.Malmerberg,
L.Johansson,
R.Andersson,
R.Bosman,
S.Carbajo,
E.Claesson,
C.E.Conrad,
P.Dahl,
G.Hammarin,
M.S.Hunter,
C.Li,
S.Lisova,
D.Milathianaki,
J.Robinson,
C.Safari,
A.Sharma,
G.Williams,
C.Wickstrand,
O.Yefanov,
J.Davidsson,
D.P.DePonte,
A.Barty,
G.Brändén,
R.Neutze.
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ABSTRACT
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Serial protein crystallography was developed at X-ray free-electron lasers
(XFELs) and is now also being applied at storage ring facilities. Robust
strategies for the growth and optimization of microcrystals are needed to
advance the field. Here we illustrate a generic strategy for recovering
high-density homogeneous samples of microcrystals starting from conditions known
to yield large (macro) crystals of the photosynthetic reaction center of
Blastochloris viridis (RCvir). We first crushed these crystals prior
to multiple rounds of microseeding. Each cycle of microseeding facilitated
improvements in the RCvirserial femtosecond crystallography (SFX)
structure from 3.3-Å to 2.4-Å resolution. This approach may allow known
crystallization conditions for other proteins to be adapted to exploit novel
scientific opportunities created by serial crystallography.
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Links
