PDBsum entry 5lxr

RNA binding protein

PDB id: 5lxr

Contents

Protein chains

80 a.a.

40 a.a.

Metals

_CL

_BR ×4

_SM ×3

Waters ×64

PDB id:

5lxr

Name:

RNA binding protein

Title:

Structure of the minimal rbm7 - zcchc8 complex

Structure:

RNA-binding protein 7. Chain: a. Synonym: RNA-binding motif protein 7. Engineered: yes. Zinc finger cchc domain-containing protein 8. Chain: b. Synonym: tramp-like complex RNA-binding factor zcchc8. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: rbm7. Expressed in: escherichia coli. Expression_system_taxid: 469008. Gene: zcchc8.

Resolution:

2.00Å R-factor: 0.202 R-free: 0.229

Authors:

S.Falk,K.Finogenova,C.Benda,E.Conti

Key ref:

S.Falk et al. (2016). Structure of the RBM7-ZCCHC8 core of the NEXT complex reveals connections to splicing factors. Nat Commun, 7, 13573. PubMed id: 27905398

Date:

22-Sep-16 Release date: 14-Dec-16

Protein chain

Q9Y580  (RBM7_HUMAN) -  RNA-binding protein 7 from Homo sapiens

Seq: 266 a.a.

Struc: 80 a.a.

Protein chain

Q6NZY4  (ZCHC8_HUMAN) -  Zinc finger CCHC domain-containing protein 8 from Homo sapiens

Seq: 707 a.a.

Struc: 40 a.a.

Nat Commun 7:13573 (2016)

PubMed id: 27905398

Structure of the RBM7-ZCCHC8 core of the NEXT complex reveals connections to splicing factors.

S.Falk, K.Finogenova, M.Melko, C.Benda, S.Lykke-Andersen, T.H.Jensen, E.Conti.

ABSTRACT

The eukaryotic RNA exosome participates extensively in RNA processing and degradation. In human cells, three accessory factors (RBM7, ZCCHC8 and hMTR4) interact to form the nuclear exosome targeting (NEXT) complex, which directs a subset of non-coding RNAs for exosomal degradation. Here we elucidate how RBM7 is incorporated in the NEXT complex. We identify a proline-rich segment of ZCCHC8 as the interaction site for the RNA-recognition motif (RRM) of RBM7 and present the crystal structure of the corresponding complex at 2.0 Å resolution. On the basis of the structure, we identify a proline-rich segment within the splicing factor SAP145 with strong similarity to ZCCHC8. We show that this segment of SAP145 not only binds the RRM region of another splicing factor SAP49 but also the RRM of RBM7. These dual interactions of RBM7 with the exosome and the spliceosome suggest a model whereby NEXT might recruit the exosome to degrade intronic RNAs.