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PDBsum entry 5lxm
Go to PDB code: 
protein ligands metals Protein-protein interface(s) links
Transferase PDB id
5lxm

 

 

 

 

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JSmol PyMol  
Contents
Protein chains
267 a.a.
34 a.a.
Ligands
ADP
PG4
SO4
PEG ×2
MES
P4G
Metals
_MG ×2
_CL
Waters ×77
PDB id:
5lxm
Name: Transferase
Title: Crystal structure of aurora-a bound to a hydrocarbon-stapled proteomimetic of tpx2
Structure: Aurora kinase a. Chain: a. Synonym: aurora 2,aurora/ipl1-related kinase 1,hark1,breast tumor- amplified kinase,serine/threonine-protein kinase 15,serine/threonine- protein kinase 6,serine/threonine-protein kinase aurora-a. Engineered: yes. Mutation: yes. Targeting protein for xklp2. Chain: d.
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: aurka, aik, airk1, ark1, aura, ayk1, btak, iak1, stk15, stk6. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Organism_taxid: 9606
Resolution:
2.08Å     R-factor:   0.202     R-free:   0.243
Authors: P.J.Mcintyre,R.Bayliss
Key ref: Y.K.Rennie et al. (2016). A TPX2 Proteomimetic Has Enhanced Affinity for Aurora-A Due to Hydrocarbon Stapling of a Helix. Acs Chem Biol, 11, 3383-3390. PubMed id: 27775325 DOI: 10.1021/acschembio.6b00727
Date:
22-Sep-16     Release date:   02-Nov-16    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
O14965  (AURKA_HUMAN) -  Aurora kinase A from Homo sapiens
Seq:
Struc: 		403 a.a.
267 a.a.*
Protein chain
Pfam   ArchSchema ?
Q9ULW0  (TPX2_HUMAN) -  Targeting protein for Xklp2 from Homo sapiens
Seq:
Struc: 		 
Seq:
Struc: 		747 a.a.
34 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 5 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: Chain A: E.C.2.7.11.1  - non-specific serine/threonine protein kinase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction:
1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H+
2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H+
L-seryl-[protein]
 + ATP
 = O-phospho-L-seryl-[protein]
Bound ligand (Het Group name = ADP)
corresponds exactly 		+ ADP
 + H(+)
L-threonyl-[protein]
 + ATP
 = O-phospho-L-threonyl-[protein]
Bound ligand (Het Group name = ADP)
corresponds exactly 		+ ADP
 + H(+)
Molecule diagrams generated from .mol files obtained from the KEGG ftp site

 

 
    reference    
 
 
DOI no: 10.1021/acschembio.6b00727 Acs Chem Biol 11:3383-3390 (2016)
PubMed id: 27775325  
 
 
A TPX2 Proteomimetic Has Enhanced Affinity for Aurora-A Due to Hydrocarbon Stapling of a Helix.
Y.K.Rennie, P.J.McIntyre, T.Akindele, R.Bayliss, A.G.Jamieson.
 
  ABSTRACT  
 
No abstract given.

 

 

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