PDBsum entry 5l6d

Transferase

PDB id: 5l6d

Contents

Protein chains

199 a.a.

238 a.a.

Ligands

SAH

ACT

Metals

_MG

Waters ×399

PDB id:

5l6d

Name:

Transferase

Title:

Crystal structure of the human mettl3-mettl14 complex bound to sah

Structure:

N6-adenosine-methyltransferase 70 kda subunit. Chain: a. Fragment: unp residues 354-580. Synonym: mt-a70,methyltransferase-like protein 3. Engineered: yes. N6-adenosine-methyltransferase subunit mettl14. Chain: b. Fragment: unp residues 107-395. Synonym: methyltransferase-like protein 14.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: mettl3, mta70. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Expression_system_cell_line: sf9. Gene: mettl14, kiaa1627. Expression_system_cell_line: sf9

Resolution:

1.85Å R-factor: 0.159 R-free: 0.192

Authors:

P.Sledz,M.Jinek

Key ref:

P.Sledz and m.jinek (2016). Structural insights into the molecular mechanism of t m(6)a writer complex.. Elife, 5, . PubMed id: 27627798

Date:

29-May-16 Release date: 12-Oct-16

Protein chain

Q86U44  (MTA70_HUMAN) -  N6-adenosine-methyltransferase catalytic subunit from Homo sapiens

Seq: 580 a.a.

Struc: 199 a.a.

Protein chain

Q9HCE5  (MET14_HUMAN) -  N6-adenosine-methyltransferase non-catalytic subunit from Homo sapiens

Seq: 456 a.a.

Struc: 238 a.a.

Enzyme reactions

• Enzyme class 1: Chain A: E.C.2.1.1.348 - mRNA m(6)A methyltransferase.
• Reaction: an adenosine in mRNA + S-adenosyl-L-methionine = an N₆-methyladenosine in mRNA + S-adenosyl-L-homocysteine + H⁺

adenosine in mRNA + S-adenosyl-L-methionine = N(6)-methyladenosine in mRNA + S-adenosyl-L-homocysteine + H(+) (Bound ligand (Het Group name = SAH) corresponds exactly)

• Enzyme class 2: Chain B: E.C.2.1.1.62 - mRNA (2'-O-methyladenosine-N(6)-)-methyltransferase.
• Reaction: a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyladenosine) in mRNA + S-adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-(N(6),2'-O-dimethyladenosine) in mRNA + S-adenosyl-L-homocysteine + H⁺

5'-end (N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyladenosine) in mRNA + S-adenosyl-L-methionine = 5'-end (N(7)-methyl 5'-triphosphoguanosine)-(N(6),2'-O-dimethyladenosine) in mRNA + S-adenosyl-L-homocysteine + H(+) (Bound ligand (Het Group name = SAH) corresponds exactly)

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site