PDBsum entry 5khs

Membrane protein

PDB id: 5khs

Contents

Protein chains

853 a.a.

PDB id:

5khs

Name:

Membrane protein

Title:

Crystal structures of the burkholderia multivorans hopanoid transporter hpnn

Structure:

Putative rnd superfamily efflux pump membrane protein. Chain: b, a. Engineered: yes. Mutation: yes

Source:

Burkholderia multivorans. Organism_taxid: 87883. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

3.76Å R-factor: 0.265 R-free: 0.307

Authors:

C.-C.Su,E.W.Yu

Key ref:

N.Kumar et al. (2017). Crystal structures of theBurkholderia multivoranshopanoid transporter HpnN. Proc Natl Acad Sci U S A, 114, 6557-6562. PubMed id: 28584102

Date:

15-Jun-16 Release date: 14-Jun-17

Protein chains

A0A0H3KP92  (A0A0H3KP92_BURM1) -  Hopanoid transporter HpnN from Burkholderia multivorans (strain ATCC 17616 / 249)

Seq: 877 a.a.

Struc: 853 a.a.*

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.?

Proc Natl Acad Sci U S A 114:6557-6562 (2017)

PubMed id: 28584102

Crystal structures of theBurkholderia multivoranshopanoid transporter HpnN.

N.Kumar, C.C.Su, T.H.Chou, A.Radhakrishnan, J.A.Delmar, K.R.Rajashankar, E.W.Yu.

ABSTRACT

Strains of theBurkholderia cepaciacomplex (Bcc) are Gram-negative opportunisitic bacteria that are capable of causing serious diseases, mainly in immunocompromised individuals. Bcc pathogens are intrinsically resistant to multiple antibiotics, including β-lactams, aminoglycosides, fluoroquinolones, and polymyxins. They are major pathogens in patients with cystic fibrosis (CF) and can cause severe necrotizing pneumonia, which is often fatal. Hopanoid biosynthesis is one of the major mechanisms involved in multiple antimicrobial resistance of Bcc pathogens. ThehpnNgene ofB. multivoransencodes an integral membrane protein of the HpnN family of transporters, which is responsible for shuttling hopanoids to the outer membrane. Here, we report crystal structures ofB. multivoransHpnN, revealing a dimeric molecule with an overall butterfly shape. Each subunit of the transporter contains 12 transmembrane helices and two periplasmic loops that suggest a plausible pathway for substrate transport. Further analyses indicate that HpnN is capable of shuttling hopanoid virulence factors from the outer leaflet of the inner membrane to the periplasm. Taken together, our data suggest that the HpnN transporter is critical for multidrug resistance and cell wall remodeling inBurkholderia.