PDBsum entry 5kd2

Hydrolase

PDB id: 5kd2

Contents

Protein chain

591 a.a.

Ligands

EDO ×5

Metals

_ZN

Waters ×373

PDB id:

5kd2

Name:

Hydrolase

Title:

Bt_4244 metallopeptidase from bacteroides thetaiotaomicron

Structure:

Metallopeptidase. Chain: a. Fragment: unp residues 274-857. Engineered: yes. Mutation: yes

Source:

Bacteroides thetaiotaomicron. Organism_taxid: 226186. Strain: atcc 29148 / dsm 2079 / nctc 10582 / e50 / vpi-5482. Gene: bt_4244. Expressed in: escherichia coli 'bl21-gold(de3)plyss ag'. Expression_system_taxid: 866768

Resolution:

2.15Å R-factor: 0.171 R-free: 0.210

Authors:

I.Noach,A.B.Boraston

Key ref:

I.Noach et al. (2017). Recognition of protein-linked glycans as a determinant of peptidase activity. Proc Natl Acad Sci U S A, 114, E679. PubMed id: 28096352 DOI: 10.1073/pnas.1615141114

Date:

07-Jun-16 Release date: 11-Jan-17

Protein chain

Q89ZX7  (Q89ZX7_BACTN) -  Peptidase M60 domain-containing protein from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50)

Seq: 857 a.a.

Struc: 591 a.a.*

* PDB and UniProt seqs differ at 8 residue positions (black crosses)

DOI no: 10.1073/pnas.1615141114

Proc Natl Acad Sci U S A 114:E679 (2017)

PubMed id: 28096352

Recognition of protein-linked glycans as a determinant of peptidase activity.

I.Noach, E.Ficko-Blean, B.Pluvinage, C.Stuart, M.L.Jenkins, D.Brochu, N.Buenbrazo, W.Wakarchuk, J.E.Burke, M.Gilbert, A.B.Boraston.

ABSTRACT

No abstract given.