PDBsum entry 5k5q

Transcription/DNA

PDB id: 5k5q

Contents

Protein chains

(+ 0 more) 91 a.a.

DNA/RNA

Ligands

PO4 ×2

Waters ×5

PDB id:

5k5q

Name:

Transcription/DNA

Title:

Structure of aspa-DNA complex: novel centromere bindng protein- centromere complex

Structure:

Aspa. Chain: c, d, a, b, e, f. Engineered: yes. DNA (32-mer). Chain: p. Engineered: yes. DNA (32-mer). Chain: n. Engineered: yes

Source:

Sulfolobus sp. Nob8h2. Organism_taxid: 84600. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Synthetic construct. Organism_taxid: 32630. Organism_taxid: 32630

Resolution:

2.65Å R-factor: 0.262 R-free: 0.280

Authors:

M.Schumacher

Key ref:

M.A.Schumacher et al. (2015). Structures of archaeal DNA segregation machinery reveal bacterial and eukaryotic linkages. Science, 349, 1120-1124. PubMed id: 26339031 DOI: 10.1126/science.aaa9046

Date:

23-May-16 Release date: 01-Jun-16

Supersedes:

4rsb

Protein chains

O93706  (O93706_9CREN) -  HTH arsR-type domain-containing protein from Sulfolobus sp. NOB8H2

Seq: 93 a.a.

Struc: 91 a.a.

DNA/RNA chains

A-A-A-T-T-G-C-T-C-T-A-T-G-T-T-A-A-T-C-G-C-A-G-A-G-C-A-T-A-T-T-T 32 bases

A-A-A-T-A-T-G-C-T-C-T-A-T-G-A-T-T-A-A-C-A-T-A-G-A-G-C-A-A-T-T-T 32 bases

DOI no: 10.1126/science.aaa9046

Science 349:1120-1124 (2015)

PubMed id: 26339031

Structures of archaeal DNA segregation machinery reveal bacterial and eukaryotic linkages.

M.A.Schumacher, N.K.Tonthat, J.Lee, F.A.Rodriguez-Castañeda, N.B.Chinnam, A.K.Kalliomaa-Sanford, I.W.Ng, M.T.Barge, P.L.Shaw, D.Barillà.

ABSTRACT

Although recent studies have provided a wealth of information about archaeal biology, nothing is known about the molecular basis of DNA segregation in these organisms. Here, we unveil the machinery and assembly mechanism of the archaeal Sulfolobus pNOB8 partition system. This system uses three proteins: ParA; an atypical ParB adaptor; and a centromere-binding component, AspA. AspA utilizes a spreading mechanism to create a DNA superhelix onto which ParB assembles. This supercomplex links to the ParA motor, which contains a bacteria-like Walker motif. The C domain of ParB harbors structural similarity to CenpA, which dictates eukaryotic segregation. Thus, this archaeal system combines bacteria-like and eukarya-like components, which suggests the possible conservation of DNA segregation principles across the three domains of life.