PDBsum entry 5k2c

Membrane protein

PDB id: 5k2c

Contents

Protein chain

396 a.a.

Ligands

ZMA

CLR ×3

OLC ×10

OLA ×11

PEG

Metals

_NA

Waters ×106

PDB id:

5k2c

Name:

Membrane protein

Title:

1.9 angstrom a2a adenosine receptor structure with sulfur sad phasing and phase extension using xfel data

Structure:

Adenosine receptor a2a/soluble cytochrome b562 chimera. Chain: a. Synonym: cytochrome b-562. Engineered: yes

Source:

Homo sapiens, escherichia coli. Human. Organism_taxid: 9606, 562. Gene: adora2a, adora2, cybc. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108

Resolution:

1.90Å R-factor: 0.174 R-free: 0.208

Authors:

A.Batyuk,L.Galli,A.Ishchenko,G.W.Han,C.Gati,P.Popov,M.-Y.Lee, B.Stauch,T.A.White,A.Barty,A.Aquila,M.S.Hunter,M.Liang,S.Boutet, M.Pu,Z.-J.Liu,G.Nelson,D.James,C.Li,Y.Zhao,J.C.H.Spence,W.Liu, P.Fromme,V.Katritch,U.Weierstall,R.C.Stevens,V.Cherezov,Gpcr Network (Gpcr)

Key ref:

A.Batyuk et al. (2016). Native phasing of x-ray free-electron laser data for a G protein-coupled receptor. Sci Adv, 2, e1600292. PubMed id: 27679816 DOI: 10.1126/sciadv.1600292

Date:

18-May-16 Release date: 21-Sep-16

Protein chain

P0ABE7  (C562_ECOLX) -  Soluble cytochrome b562 from Escherichia coli

Seq: 128 a.a.

Struc: 396 a.a.*

Protein chain

P29274  (AA2AR_HUMAN) -  Adenosine receptor A2a from Homo sapiens

Seq: 412 a.a.

Struc: 396 a.a.*

* PDB and UniProt seqs differ at 112 residue positions (black crosses)

DOI no: 10.1126/sciadv.1600292

Sci Adv 2:e1600292 (2016)

PubMed id: 27679816

Native phasing of x-ray free-electron laser data for a G protein-coupled receptor.

A.Batyuk, L.Galli, A.Ishchenko, G.W.Han, C.Gati, P.A.Popov, M.Y.Lee, B.Stauch, T.A.White, A.Barty, A.Aquila, M.S.Hunter, M.Liang, S.Boutet, M.Pu, Z.J.Liu, G.Nelson, D.James, C.Li, Y.Zhao, J.C.Spence, W.Liu, P.Fromme, V.Katritch, U.Weierstall, R.C.Stevens, V.Cherezov.

ABSTRACT

No abstract given.